Guidance for interpreting experiments on DNA repair

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In human cells, both normal metabolic activities and environmental factors such as UV light and radiation can cause DNA damage.


It is important to try to capture the specific type of DNA repair mechanism that is being carried out by a gene product, as some proteins specifically carry out one type of repair.

Example:

POL1 could be annotated to NOT nucleotide excision repair (PMID 2651896)

POL1 should be annotated to the more specific double-stranded break repair (PMID 10025407)


Nucleotide excision repair (NER) recognizes bulky, helix-distorting lesions, that could be caused by damage by experiments applying:

  • UV irradiation (resulting in pyrimidine dimers and 6-4 photoproducts)
  • chemicals (resulting in intrastrand cross-links and bulky adducts).


N.B. this is a stub guidance page, please add further information.