2012 PAINT

Preliminary agenda

DAY 1 AM

• Introduction to evolution (Paul)
• PAINT: overview and general utilities (Huaiyu)

DAY 1 PM

• Walk though an example: CCS
• The goal is to get everyone familiar with the PAINT tool.

BREAK

• First common example (TBD - easy)
  • MF annotation
  • MF discussion

• • CC annotation
  • CC discussion

• • BP annotation
  • BP discussion

---

DAY 2 AM

• Walk through a more complicated example

We need to define types of examples with specific issues:

• • Families where a member has lost a function

WE NEED TO START A TABLE with types

Part of BP discussion:

• Annotations to be cautious about (Pascale)
  • Regulation of tyrosine hydroxylase activity
  • Activation of transcription factors, including production of downstream proteins (interleukins)

---

DAY 3 & DAY 4

• Everyone works on example trees.
• One family each half day, 4 examples total
• Leave an hour at the end of each half day for wrap-up discussion. One participant should present the annotation and lead the discussion.

---

List of possible examples for the workshop

• PTHR19443 (Hexokinase) - A classic example of protein families involved in metabolic pathways.
• PTHR11537 (Kv channela) - A typical example of families of ion channels, transporters and receptors with lots of gene duplications.
• PTHR19957 (Syntaxin) - similar as above.
• PTHR11256 (blc-2) - Too many GO terms. How to prioritize?
• PTHR11354 (Ubiquitin protein ligase)
• PTHR11829 (Transcription factor)