2012 PAINT
PAINT User Guide: http://wiki.geneontology.org/index.php/PAINT_User_Guide
Preliminary agenda
DAY 1 AM
- Introduction to evolution of gene families and function (Paul)
- PAINT: overview and general utilities (Huaiyu)
DAY 1 PM
- Walk though an example: PTHR10003 (CCS)
- The goal is to get everyone familiar with the PAINT tool.
BREAK
- First common example: PTHR19443 (Hexokinase)
- MF annotation
- MF discussion
- CC annotation
- CC discussion
- BP annotation
- BP discussion
DAY 2 AM
- Walk through more complicated examples
- PTHR11537 (Kv channel)
- PTHR11254 (Ubiquitin protein ligase)
- PTHR19384 Nitric oxide synthase
- Excessive downstream processes
- Dinner at Huaiyu's house in Palo Alto. Please plan accordingly.
DAY 3 & DAY 4
- Everyone works on example trees.
- One family each half day, 4 examples total
- Leave an hour at the end of each half day for wrap-up discussion. One participant should present the annotation and lead the discussion.
List of possible examples for the workshop
- Gene duplications
- PTHR19957 (Syntaxin) - similar as above.
- Transcription factors
- PTHR11829 (Transcription factor)
- Very complicated families
- PTHR11256 (bcl-2) - Too many GO terms. How to prioritize?
- ??
- PTHR11406 (Phosphofructokinase)
- PTHR11361? Example of loss of function / stop propagation / add new function without loss
Pascale
- PTHR10454 Caspase family
- PMID: 23070001
- PMID: 22949111
- PMID: 22606010
- http://en.wikipedia.org/wiki/Caspase
- PTHR10454 Caspase family
Part of BP discussion:
- Annotations to be cautious about (Pascale)
- Regulation of tyrosine hydroxylase activity
- Activation of transcription factors, including production of downstream proteins (interleukins)