Difference between revisions of "Annotation Conf. Call 2015-10-27"

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(Annotation Consistency Exercise)
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==Annotation Consistency Exercise==
 
==Annotation Consistency Exercise==
 
Annotation consistency paper picked by ZFIN- PMID:24430697
 
Annotation consistency paper picked by ZFIN- PMID:24430697
 +
 +
'''Choice of paper-motivation''': discussing the use of IGI as evidence code.
 +
When should we use IGI?
 +
:Indented line - mutation in gene A is rescued by a mutation in gene B
 +
:Indented line - mutation in gene A is rescued by over-expression in gene
 +
In this paper, how do we capture
 +
:Indented line - the repression of transcriptional activation activity of tbx5a by kctd10?
 +
:Indented line - the information about HAS2
 +
 +
'''Summary of the paper''' (PMID:24430697)
 +
- mutants in kctd10 show heart defects (heart looping, atrioventricular canal development)
 +
Note: phenotypes determined by
 +
:Indented line - analysis of heart morphology
 +
:Indented line - gene expression analysis of genes known to be expressed in specific part of the heart).
 +
[Fig 1, 2, 3]
 +
 +
- downregulation of tbx5a or has 2 (using MO) rescue kctd10 mutant heart phenotypes.
 +
[Fig 4]
 +
 +
- in vitro experiments [Fig5]:
 +
:Indented line - luciferase reporter assay
 +
kctd10 inhibits tbx5 transcriptional activity
 +
:Indented line - Co-IP ; Pull down assay:
 +
tbx5a and kctd10 physically interact with each other.
  
 
==Minutes==
 
==Minutes==
  
 
[[Category:Annotation Working Group]]
 
[[Category:Annotation Working Group]]

Revision as of 16:13, 26 October 2015

Meeting URL: https://bluejeans.com/993661940

Agenda

Annotation Consistency Exercise

Annotation consistency paper picked by ZFIN- PMID:24430697

Choice of paper-motivation: discussing the use of IGI as evidence code. When should we use IGI?

Indented line - mutation in gene A is rescued by a mutation in gene B
Indented line - mutation in gene A is rescued by over-expression in gene

In this paper, how do we capture

Indented line - the repression of transcriptional activation activity of tbx5a by kctd10?
Indented line - the information about HAS2

Summary of the paper (PMID:24430697) - mutants in kctd10 show heart defects (heart looping, atrioventricular canal development) Note: phenotypes determined by

Indented line - analysis of heart morphology
Indented line - gene expression analysis of genes known to be expressed in specific part of the heart).

[Fig 1, 2, 3]

- downregulation of tbx5a or has 2 (using MO) rescue kctd10 mutant heart phenotypes. [Fig 4]

- in vitro experiments [Fig5]:

Indented line - luciferase reporter assay

kctd10 inhibits tbx5 transcriptional activity

Indented line - Co-IP ; Pull down assay:

tbx5a and kctd10 physically interact with each other.

Minutes