Annotation Conf. Call 2015-10-27: Difference between revisions
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==Annotation Consistency Exercise== | ==Annotation Consistency Exercise== | ||
Annotation consistency paper picked by ZFIN- PMID:24430697 | Annotation consistency paper picked by ZFIN- PMID:24430697 | ||
'''Choice of paper-motivation''': discussing the use of IGI as evidence code. | |||
When should we use IGI? | |||
:Indented line - mutation in gene A is rescued by a mutation in gene B | |||
:Indented line - mutation in gene A is rescued by over-expression in gene | |||
In this paper, how do we capture | |||
:Indented line - the repression of transcriptional activation activity of tbx5a by kctd10? | |||
:Indented line - the information about HAS2 | |||
'''Summary of the paper''' (PMID:24430697) | |||
- mutants in kctd10 show heart defects (heart looping, atrioventricular canal development) | |||
Note: phenotypes determined by | |||
:Indented line - analysis of heart morphology | |||
:Indented line - gene expression analysis of genes known to be expressed in specific part of the heart). | |||
[Fig 1, 2, 3] | |||
- downregulation of tbx5a or has 2 (using MO) rescue kctd10 mutant heart phenotypes. | |||
[Fig 4] | |||
- in vitro experiments [Fig5]: | |||
:Indented line - luciferase reporter assay | |||
kctd10 inhibits tbx5 transcriptional activity | |||
:Indented line - Co-IP ; Pull down assay: | |||
tbx5a and kctd10 physically interact with each other. | |||
==Minutes== | ==Minutes== | ||
[[Category:Annotation Working Group]] | [[Category:Annotation Working Group]] |
Revision as of 19:13, 26 October 2015
Meeting URL: https://bluejeans.com/993661940
Agenda
Annotation Consistency Exercise
Annotation consistency paper picked by ZFIN- PMID:24430697
Choice of paper-motivation: discussing the use of IGI as evidence code. When should we use IGI?
- Indented line - mutation in gene A is rescued by a mutation in gene B
- Indented line - mutation in gene A is rescued by over-expression in gene
In this paper, how do we capture
- Indented line - the repression of transcriptional activation activity of tbx5a by kctd10?
- Indented line - the information about HAS2
Summary of the paper (PMID:24430697) - mutants in kctd10 show heart defects (heart looping, atrioventricular canal development) Note: phenotypes determined by
- Indented line - analysis of heart morphology
- Indented line - gene expression analysis of genes known to be expressed in specific part of the heart).
[Fig 1, 2, 3]
- downregulation of tbx5a or has 2 (using MO) rescue kctd10 mutant heart phenotypes. [Fig 4]
- in vitro experiments [Fig5]:
- Indented line - luciferase reporter assay
kctd10 inhibits tbx5 transcriptional activity
- Indented line - Co-IP ; Pull down assay:
tbx5a and kctd10 physically interact with each other.