Annotation Conf. Call 2015-10-27

From GO Wiki
Jump to navigation Jump to search
The printable version is no longer supported and may have rendering errors. Please update your browser bookmarks and please use the default browser print function instead.

Meeting URL: https://bluejeans.com/993661940

Agenda

Annotation Consistency Exercise

Annotation consistency paper picked by ZFIN- PMID:24430697

Choice of paper-motivation: discussing the use of IGI as evidence code. When should we use IGI?

Indented line - mutation in gene A is rescued by a mutation in gene B
Indented line - mutation in gene A is rescued by over-expression in gene

In this paper, how do we capture

Indented line - the repression of transcriptional activation activity of tbx5a by kctd10?
Indented line - the information about HAS2

Summary of the paper (PMID:24430697) - mutants in kctd10 show heart defects (heart looping, atrioventricular canal development) Note: phenotypes determined by

Indented line - analysis of heart morphology
Indented line - gene expression analysis of genes known to be expressed in specific part of the heart).

[Fig 1, 2, 3]

- downregulation of tbx5a or has 2 (using MO) rescue kctd10 mutant heart phenotypes. [Fig 4]

- in vitro experiments [Fig5]:

Indented line - luciferase reporter assay

kctd10 inhibits tbx5 transcriptional activity

Indented line - Co-IP ; Pull down assay:

tbx5a and kctd10 physically interact with each other.

Minutes