Annotation Conf. Call 2015-11-24

Agenda

Annotation Consistency Exercise

Annotation consistency paper picked by TAIR: The MYB36 transcription factor orchestrates Casparian strip formation.

Choice of paper-motivation: capturing downstream targets and their resulting effects, importance of reading term definitions

Annotations requested to be restricted to Figures 1-4 only, to keep discussion manageable.

Summary of the paper

MYB36 is a transcription factor that controls where the machinery for deposition/biosynthesis of lignin is expressed The location of the machinery determines where the Casparian strip can form.

Figure 1.

 leaf ionome mutant vs. wt
 root hair phenotype mutant vs. wt
 endodermal localization of MYB36 expression

Figure 2.

 Loss of Casparian strip and disruption of the apoplastic barrier in myb36 mutants.

Figure 3.

 Identifying target genes of MYB36 - qPCR
 MYB36 binds specific target gene promoters - ChIP
 

Figure 4.

 CASP1 localization
 Ectopic Casparian strip formation with induced ectopic expression of MYB36

Annotations: Annotations received from five groups, TAIR included. (MGI, Pombase, WormBase, UCL)