Annotation Conf. Call 2016-06-28

Bluejeans URL

https://bluejeans.com/993661940

Agenda

Protein Binding Annotations

• Review Protein Binding Annotation Guidelines
• Use of With/From vs Annotation Extensions
• Annotation of Direct vs Indirect Binding
  • Types of Experiments Used to Inform Physical Interactions
    • Yeast two hybrid
    • CoIP followed by Western blot
    • CoIP followed by mass spec
    • GST pull down using recombinant proteins
    • GST pull down using lysate
• Use Cases
  • GO:0030674 protein binding, bridging - The binding activity of a molecule that brings together two or more protein molecules, or a protein and another macromolecule or complex, through a selective, non-covalent, often stoichiometric interaction, permitting those molecules to function in a coordinated way. Source: GOC:mah, GOC:vw, GOC:bf
    • Evidence codes: IDA and IPI
    • IPI has With/From, AE, or both With/From and AE
    • AE uses has_input or has_direct_input for relation
  • GO:0043495 protein anchor - Interacting selectively and non-covalently with both a protein or protein complex and a membrane, in order to maintain the localization of the protein at a specific location on the membrane. Source: GOC:go_curators
    • Evidence codes: IDA, IGI, IMP, IPI
    • IPI only uses With/From