Annotation Conf. Call 2016-06-28
Bluejeans URL
https://bluejeans.com/993661940
Agenda
Annotation Consistency Exercise
Protein Binding Annotations
- Review Protein Binding Annotation Guidelines
- Use of With/From vs Annotation Extensions
- Annotation of Direct vs Indirect Binding
- Types of Experiments Used to Inform Physical Interactions
- Yeast two hybrid
- CoIP followed by Western blot
- CoIP followed by mass spec
- GST pull down using recombinant proteins
- GST pull down using lysate
- Types of Experiments Used to Inform Physical Interactions
- Use Cases
- GO:0030674 protein binding, bridging - The binding activity of a molecule that brings together two or more protein molecules, or a protein and another macromolecule or complex, through a selective, non-covalent, often stoichiometric interaction, permitting those molecules to function in a coordinated way. Source: GOC:mah, GOC:vw, GOC:bf
- Evidence codes: IDA and IPI
- IPI has With/From, AE, or both With/From and AE
- AE uses has_input or has_direct_input for relation
- GO:0043495 protein anchor - Interacting selectively and non-covalently with both a protein or protein complex and a membrane, in order to maintain the localization of the protein at a specific location on the membrane. Source: GOC:go_curators
- Evidence codes: IDA, IGI, IMP, IPI
- IPI only uses With/From
- GO:0030674 protein binding, bridging - The binding activity of a molecule that brings together two or more protein molecules, or a protein and another macromolecule or complex, through a selective, non-covalent, often stoichiometric interaction, permitting those molecules to function in a coordinated way. Source: GOC:mah, GOC:vw, GOC:bf