Annotation Conf. Call September 24th, 2013

Annotation extension exercises

Paper 1: PMID 17620096 (Title: Human S100A15 splice variants are differentially expressed in inflammatory skin diseases and regulated through Th1 cytokines and calcium.)

http://www.ncbi.nlm.nih.gov/pubmed/17620096

Annotation Extension to be discussed for the cytoplasm annotation for S100A14.

DB (Col 2)	GO ID (Col 5)	ev.code	Reference (Col 6)	Extension (Col 16)
S100A14, Q9HCY8	GO:0005737 (cytoplasm)	IDA	PMID:17620096	part_of: CL:0000362 (epidermal cell)

Evidence is from Figure 3 and text on page 687: "hS100A15 is predominantly expressed in the epidermis of psoriatic and atopic skin Because psoriasis and atopic eczema are characterized by morphological changes in both dermis and epidermis, we investigated the distribution of the hS100A15 transcripts in normal and diseased skin by in situ hybridization. Both S100A15 isoforms were detected mainly in the epidermis of normal and inflamed skin and showed a similar distribu- tion pattern. In line with RT-PCR analysis, the expression of S100A15-S was less pronounced than S100A15-L. Within the faint staining of healthy skin, hS100A15-L transcripts were present in the cytoplasm of the basal epidermal layer (Fig. 3, arrow), whereas the differentiated epidermal layers showed weak probe reactivity to hS100A15-L. "

Paper 2: PMID 9799565 (Title: Role of human CYP4F2 in hepatic catabolism of the proinflammatory agent leukotriene B4.)

http://www.ncbi.nlm.nih.gov/pubmed/9799565

Annotation Extension to be discussed for the leukotriene-B4 20-monooxygenase activity annotation for CYP4F2.

DB (Col 2)	GO ID (Col 5)	ev.code	Reference (Col 6)	Extension (Col 16)
CYP4F2, P78329	GO:0050051 (leukotriene-B4 20-monooxygenase activity)	IDA	PMID:9799565	occurs_in: UBERON:0002107 (liver)

Evidence is from Table III

Paper 3: PMID 23437011 (Title: The Caenorhabditis elegans JNK signaling pathway activates expression of stress response genes by derepressing the Fos/HDAC repressor complex.)

http://www.ncbi.nlm.nih.gov/pubmed/23437011

Annotation Extension to be discussed for