Annotation Extension Relation:dependent on
Definition
Identifies a requirement under which the ontology term is observed to apply to the annotated gene product.
Child terms
requires_target_sequence_feature
Scope of use
Domain
BFO:0000001 ! entity (Biological Process, Molecular Function, Cellular Component)
Range
ENTITY_UNION:0000007 ! chemical, gene product, complex, cellular component, biological process, molecular function, modification or sequence feature
Annotation Extension Usage Examples
IAP2-dependent regulation of NF-kB activity:
Statement from paper:
To test whether RIP3 and RIP4 have to be ubiquitinated by cIAP1/2 in order to mediate NF-kB activation, we compared RIP-mediated NF-kB luciferase reporter activity when ectopically expressed in HEK293T cells in the presence or absence of the IAP inhibitor BV6, a treatment that induces rapid auto-ubiquitination and degradation of endogenous cIAP1/2[41]. As shown in Figure 4A, BV6 treatment greatly impaired TNF and RIP1–RIP4-induced NF-kB activation but had no impact on TAK1-mediated NF-kB induction (Figure 4A). Those results, which indicate that cIAP1/2 act upstream of TAK1, are consistent with a role for cIAP1/2 as E3 ligases regulating RIP1–4-mediated activation of NF-kB.
Gene Name (col 2) | GO ID (col 5) | Reference (col 6) | Evidence (col 7) | Annotation Extension (col 16) |
---|---|---|---|---|
Q9Y572 RIP3 | GO:0051092 positive regulation of NF-kappaB transcription factor activity | PMID:21931591 | IDA |
dependent_on(UniProtKB:Q13490 IAP2) |
Two proteins required for an activity:
Statement from paper:
To test the RuvA and RuvB proteins for DNA helicase activity, we used a simple gel electrophoretic assay which measures the displacement of a short 32P-labeled oligonucleotide (66nt long) from single-stranded circular omegaX174 DNA. Purified RuvA and RuvB catalyzed the unwinding of the oligonucleotide from the single-stranded circle (Fig.1,lane e). Neither RuvA (lanes c and d) nor RuvB (lanes f and g) alone were capable of unwinding, even at much higher protein concentrations.
Gene Name (col 2) | GO ID (col 5) | Reference (col 6) | Evidence (col 7) | Annotation Extension (col 16) |
---|---|---|---|---|
P0A809 ruvA | GO:0004003 ATP-dependent DNA helicase activity | PMID:8433990 | IDA |
dependent_on(UniProtKB:P0A812 ruvB) |
Gene Name (col 2) | GO ID (col 5) | Reference (col 6) | Evidence (col 7) | Annotation Extension (col 16) |
---|---|---|---|---|
P0A812 ruvB | GO:0004003 ATP-dependent DNA helicase activity | PMID:8433990 | IDA |
dependent_on(UniProtKB:P0A809 ruvA) |
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