Annotation Extension Relation:has direct input

Definition

Identifies an entity directly affected (bound, transported, modified, consumed or destroyed) by the gene product's participation in MF or BP.

Child terms

• None

Annotation Extension Usage Examples

Transcription factor binding to potential target sequences in vitro:

Statement from paper:

By performing gel electrophoretic mobility shift assays, we could detect efficient binding of ATF-2 to the elements found in the bec-1 and lgg-1 promoters (Fig. 5C).

(Note that binding to lin-48 is also reported as a positive control).

Gene Name (col 2)	GO ID (col 5)	Reference (col 6)	Evidence (col 7)	Annotation Extension (col 16)
WBGene00000220	GO:0001012 RNA polymerase II regulatory region DNA binding	PMID:21502138	IDA	has_direct_input(WB:WBGene00000247 bec-1)|has_direct_input(WB:WBGene00002980 lgg-1)|has_direct_input(WB:WBGene00003033 lin-48)

Targets of protein kinase activity:

Statements from paper:

A final line of evidence that BAF-1 is a substrate for VRK-1 was obtained with purified recombinant proteins, which demonstrated that BAF-1 was phosphorylated by VRK-1 in vitro (Figure 4H, lower panel). In addition, VRK-1 was autophosphorylated (Figure 4H, upper panel).

Gene Name (col 2)	GO ID (col 5)	Reference (col 6)	Evidence (col 7)	Annotation Extension (col 16)
WBGene00017895	GO:0004672 protein kinase activity	PMID:17170708	IDA	has_direct_input(WB:WBGene00000235 baf-1)

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