Has output

Definition

Identifies an entity that is changed or created after participation in a molecular function or biological process.

Child terms

• None

Scope of use

Domain

BFO:0000007 ! process (Biological Process or Molecular Function)

Range

ENTITY_UNION:0000005 ! chemical, gene product, or complex

Annotation Extension Usage Examples

1. Product of catalytic activity

Statement from paper:

Hypoxia significantly induced PGIS expression in highly vascularized tissues, including lung, brain, and kidney (Fig. 9A). Additionally, plasma levels of 6-oxo-PGF1α were significantly increased (about 2-fold) in mice exposed to hypoxia (Fig. 9B). These findings demonstrate that hypoxia stimulates the release of PGI2 in vivo and support the contribution of vascular PGIS to this effect.

Gene Name (col 2)	GO ID (col 5)	Reference (col 6)	Evidence (col 7)	Annotation Extension (col 16)
Q16647	GO:0008116 prostaglandin-I synthase activity	PMID:21296955	IDA	has_output(CHEBI:15552 prostaglandin I2)

2. Abnormal G-CSF and chemokine production in G6PT-deficient mice

Statement from paper:

The neutropenia and stimulation of myelopoiesis in G6PT−/− mice suggest that G-CSF (20,21) levels might be elevated. During the first 2–3 weeks, when neutropenia is most severe and the elevation in CFU-G is highest, the plasma G-CSF level in G6PT−/− mice is elevated to 17 263±4917 pg/ml, compared with 10±7 pg/ml in the G6PT+/+ or G6PT+/− mice (Fig. 5A). After weaning, with the improvement in neutropenia and decline in overproduction of CFU-G, the plasma G-CSF level in G6PT-deficient mice drops significantly. However, even at 22 weeks of age, the plasma G-CSF in the G6PT−/− mice remains 12-fold higher than age-matched controls (Fig. 5A), suggesting an underlying problem with neutrophil production.

Gene Name (col 2)	GO ID (col 5)	Reference (col 6)	Evidence (col 7)	Annotation Extension (col 16)
MGI:1316650 Putative glycogen storage disease type 1b protein	GO:0001816 cytokine production	PMID:12925567	IMP	has_output(MGI:1339751 Granulocyte colony-stimulating factor)

Using examples to demonstrate Folding_and_Unfolding using the relationship activated_by

1. zinc activated ligand-gated ion channel (ZACN) is activated by Zn2+, PMID:12381728

Folded/unfolded	DB (Col 2)	Object (Col 3)	GO ID (Col 5)	Reference (Col 6)	Extension (Col 16)	Parent terms for new folded GO term
Unfolded	Q401N2	ZACN	GO:0048471 ligand-gated ion channel activity	PMID:12381728	activated_by (ChEBI:29105, zinc ions)
Folded	Q401N2	ZACN	New GO term zinc-gated ion channel activity	PMID:12381728		is_a GO:0048471 ligand-gated ion channel activity

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