Occurs in

Definition

Identifies the cell, tissue, cellular component or anatomical entity in which the molecular function or biological process occurs.

Child terms

• None.

Scope of use

Domain

BFO:0000007 ! process (Biological Process or Molecular Function)

Range

ENTITY_UNION:0000006 ! entity

Annotation Extension Usage Examples

Enhancing Molecular Function and Biological Process Annotations

Specifying the cell or tissue type in which a process happens

Examples of this usage of occurs_in can be found on the documentation page for Column_16:_Cell_Type

Specifying the subcellular location in which a process happens

1. Protein interaction at the sarcoplasmic reticulum:

Statement from paper:

Canine cardiac junctional SR vesicles were solubilized with 2% Triton X-100 and loaded on the affinity column equilibrated in EGTA-containing buffer (Fig.1). Calsequestrin-binding proteins were then eluted in buffer containing 20 mm CaCl2, a calcium concentration sufficient to disrupt all calsequestrin protein-binding interactions. Immunoblotting of the eluted fractions with triadin antibody revealed that triadin bound completely to the column and was eluted as a discrete peak in Ca2+-containing buffer (Fig. 1 A, upper)

Gene Name (col 2)	GO ID (col 5)	Reference (col 6)	Evidence (col 7)	With (col 8)	Annotation Extension (col 16)
P12637 CASQ2	GO:0005515 protein binding	PMID:9287354	IPI	P82179 TRDN	occurs_in(GO:0016529 sarcoplasmic reticulum)

2. Sequestering of calcium ion at sarcoplasmic reticulum:

Statement from paper:

To characterize the effects of the new CASQ2 mutations on SR Ca2+ handling in vivo, we performed experiments in adult rat ventricular myocytes infected with adenoviral constructs. Caffeine (10 mmol/L) was applied to myocytes expressing either WT or mutant CASQ2 to evaluate the SR Ca2+-storing capacity by measurement of fluo-3 fluorescence and of Na+/Ca2+ exchange current (INCX; Figure 4A; Table 1).21 Expression of WT increased the SR Ca2+ content by 50%, whereas CASQ2 G112+5X reduced the SR content, not only as compared with WT expression but also as compared with native cells (Ad-control).

Gene Name (col 2)	GO ID (col 5)	Reference (col 6)	Evidence (col 7)	Annotation Extension (col 16)
O14958 CASQ2	GO:0051208 sequestering of calcium ion	PMID:16908766	IMP	occurs_in(GO:0016529 sarcoplasmic reticulum), occurs_in(CL:0000746 cardiac muscle cell)

Note the use of the comma to create a compound annotation extension. The interpretation of this annotation is CASQ2 is involved in sequestering calcium ions in the sarcoplasmic reticulum of cardiac muscle cell.

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