Annotation Extension Relation:requires substance

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The annotated gene product participates in a Biological Process or executes a Molecular Function only in presence of a substance

Child terms

Scope of use


BFO:0000007 ! process (Biological Process or Molecular Function)


CHEBI:24431 ! chemical entity

Annotation Extension Usage Examples

Gene product has activity only when a particular substance is present

Statement in paper;

We investigated whether the reducing equivalents required for the presumed peroxidase activity of PrxV could be provided by the Trx system (Trx, TrxR, and NADPH) or the Grx system (Grx, GSH, GSH reductase, and NADPH). The rate of H2O2 degradation was measured by monitoring the decrease in A340 attributable to the oxidation of NADPH. PrxV catalyzed the H2O2-dependent oxidation of NADPH in the presence of the Trx system (Fig. 3)...In contrast, the Grx system did not support the H2O2-dependent oxidation of NADPH by PrxV.

Gene Name (col 2) GO ID (col 5) Reference (col 6) Evidence (col 7) Annotation Extension (col 16)
P30044-2 GO:0051920 peroxiredoxin activity PMID:10751410 IDA

requires_substance(CHEBI:15033 thioredoxin)

Protein binding is dependent on a particular substance

Statement in paper;

Changing [Ca2+]i Alters the Interaction between IQGAP1 and Calmodulin. The reduced interaction between IQGAP1 and B-Raf in cells produced by increasing [Ca2+]i suggests the following mechanism: increasing [Ca2+]i enhances the binding of calmodulin to IQGAP1 (21), which attenuates binding of B-Raf to IQGAP1. To address this possibility, we examined co-immunoprecipitation of calmodulin with IQGAP1. Consistent with our model, A23187 increases by ~3-fold the amount of calmodulin that co-immunoprecipitates with IQGAP1 (Fig. 8).

Gene Name (col 2) GO ID (col 5) Reference (col 6) Evidence (col 7) With (col 8) Annotation Extension (col 16)
P46940 GO:0005516 calmodulin binding PMID:18567582 IPI P62158 calmodulin

requires_substance(CHEBI:29108 calcium 2+)

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