Annotation Extension Relation:requires substance
The annotated gene product participates in a Biological Process or executes a Molecular Function only in presence of a substance
Scope of use
BFO:0000007 ! process (Biological Process or Molecular Function)
CHEBI:24431 ! chemical entity
The GO editors have agreed that this relation should be deprecated. The relation does not extend the meaning of the GO term, as annotation relations are meant to, but instead specifies conditions under which the annotation is true. This can not be expressed in OWL. Annotation extensions using this relation will not fold.
Annotation Extension Usage Examples
Gene product has activity only when a particular substance is present
Statement in paper;
We investigated whether the reducing equivalents required for the presumed peroxidase activity of PrxV could be provided by the Trx system (Trx, TrxR, and NADPH) or the Grx system (Grx, GSH, GSH reductase, and NADPH). The rate of H2O2 degradation was measured by monitoring the decrease in A340 attributable to the oxidation of NADPH. PrxV catalyzed the H2O2-dependent oxidation of NADPH in the presence of the Trx system (Fig. 3)...In contrast, the Grx system did not support the H2O2-dependent oxidation of NADPH by PrxV.
|Gene Name (col 2)||GO ID (col 5)||Reference (col 6)||Evidence (col 7)||Annotation Extension (col 16)|
|P30044-2||GO:0051920 peroxiredoxin activity||PMID:10751410||IDA||
Protein binding is dependent on a particular substance
Statement in paper;
Changing [Ca2+]i Alters the Interaction between IQGAP1 and Calmodulin. The reduced interaction between IQGAP1 and B-Raf in cells produced by increasing [Ca2+]i suggests the following mechanism: increasing [Ca2+]i enhances the binding of calmodulin to IQGAP1 (21), which attenuates binding of B-Raf to IQGAP1. To address this possibility, we examined co-immunoprecipitation of calmodulin with IQGAP1. Consistent with our model, A23187 increases by ~3-fold the amount of calmodulin that co-immunoprecipitates with IQGAP1 (Fig. 8).
|Gene Name (col 2)||GO ID (col 5)||Reference (col 6)||Evidence (col 7)||With (col 8)||Annotation Extension (col 16)|
|P46940||GO:0005516 calmodulin binding||PMID:18567582||IPI||P62158 calmodulin||
requires_substance(CHEBI:29108 calcium 2+)