Contributes to

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Overview

Definition

Child Terms

None

Examples of Usage

Multi-subunit complexes in which the catalytic subunit is known

Annotate the catalytic subunit, without the contributes_to qualifier.

  • eIF2: has three subunits (alpha, beta, gamma); one binds GTP; one binds RNA; the whole complex binds the ribosome (all three subunits are required for ribosome binding). So one subunit is annotated to GTP binding and one to RNA binding without qualifiers, and all three stand in the contributes_to relationship to "ribosome binding". And all three are part_of an eIF2 complex.
  • eIF2 eIF2gamma is the actual GTPase, and eIF2alpha and -beta serving accessory functions. Therefore, eIF2gamma is annotated to GTPase activity; the alpha and beta subunits are not annotated at all to GTPase activity. (See https://www.ncbi.nlm.nih.gov/pubmed/29425030)
  • ATP citrate lyase (ACL) in Arabidopsis: it is a heterooctamer, composed of two types of subunits, ACLA and ACLB in a A(4)B(4) stoichiometry. Neither of the subunits expressed alone give ACL activity, but co-expression results in ACL activity. Both subunits contribute_to the ATP citrate lyase activity. (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1595482)
  • STT3 (PMID:12887896): "dolichyl-diphosphooligosaccharide-protein glycotransferase activity": although it is a member of a complex, it is the catalytic subunit, so it should not be annotated with the "contributes_to" qualifier.

Multi-subunit complexes in which the catalytic subunit has not been characterized

Annotate all subunits, with the contributes_to qualifier.

Multi-subunit complexes in which there is no single catalytic subunit

Annotate all subunits, with the contributes_to qualifier.

  • Subunits of nuclear RNA polymerases: none of the individual subunits have RNA polymerase activity, yet all of these subunits contribute_to DNA-dependent RNA polymerase activity.

When NOT to Use 'contributes to'

  • Prkag1 (protein kinase AMP-activated non-catalytic subunit gamma 1) RGD:3388: PMID:21399626 shows that the activation loop of the kinase domain is stabilized by the regulatory domain. The paper also shows that the regulatory subunit binds ADP, so it is really the subunit with a regulatory role.
    • annotation should be to "GO:0019887 protein kinase regulator activity"; Prkag1 does NOT contribute to "AMP-activated protein kinase activity".
  • Prkaa1 (protein kinase AMP-activated catalytic subunit alpha 1) RGD:3387 is the active subunit; is should be annotated to "AMP-activated protein kinase activity", without the "contributes_to" qualifier.


Quality Control Checks

Relations Ontology

contributes to

Review Status

Last reviewed: September 9, 2019

Back to: Annotation Relations