GAF file QC

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(Created page with 'The following quality control checks should be performed before submitting a GAF file to the Gene Ontology Consortium. Remove any duplicate lines in the file (this can be easily…')
 
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Ensure all external entities referenced in the file (e.g. in the '''with/from''' column) use the correct database prefix, as specified in the [http://www.geneontology.org/doc/GO.xrf_abbs xref abbreviations file]
Ensure all external entities referenced in the file (e.g. in the '''with/from''' column) use the correct database prefix, as specified in the [http://www.geneontology.org/doc/GO.xrf_abbs xref abbreviations file]
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Check GAF based on taxon constraints - see [[GAF_Taxonomy_Reasoning]] for details. This can be done in two ways
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* Submit the GAF and then check the report some time later
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* Install the GOBO package and run the constraints locally prior to submission
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The first way is certainly easier, but the disadvantage is that the gaffes are public for a while. The second will require the help of someone experienced with installing perl modules.
[[Category:Annotation]]
[[Category:Annotation]]
[[Category:GAF]]
[[Category:GAF]]

Latest revision as of 20:09, 8 January 2010

The following quality control checks should be performed before submitting a GAF file to the Gene Ontology Consortium.

Remove any duplicate lines in the file (this can be easily accomplished by performing sort -u on Unix)

Ensure all external entities referenced in the file (e.g. in the with/from column) use the correct database prefix, as specified in the xref abbreviations file

Check GAF based on taxon constraints - see GAF_Taxonomy_Reasoning for details. This can be done in two ways

  • Submit the GAF and then check the report some time later
  • Install the GOBO package and run the constraints locally prior to submission

The first way is certainly easier, but the disadvantage is that the gaffes are public for a while. The second will require the help of someone experienced with installing perl modules.

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