Inferred from High Throughput Mutant Phenotype (HMP)

HMP: Inferred from High Throughput Mutant Phenotype

Overview

The HMP evidence code covers those cases when the function, process or cellular localization of a gene product is inferred based on differences in the function, process, or cellular localization between two different alleles of the corresponding gene in a high throughput experiment. In high throughput perturbation screens, often particular phenotypic traits will be used as readout. It is particularly important that the phenotypic output assayed directly relates to the conclusion/annotation. Curators should be particularly careful about the choice of term and satisfied that the authors have taken sufficient steps to demonstrate direct correlation. A potential source of false positives may be from the mis-association of a phenotype to gene. For RNAi studies this arises from off-target effects and with mutagenesis studies whether the genetic lesion has been mapped to the correct gene. The curator should check that the authors have taken sufficient steps to reduce misattribution of phenotypes.

The HMP evidence code is equivalent to the IMP code. The guidelines for annotating using a HMP code are the same as for the IMP code.

Evidence and Conclusion Ontology

ECO:0007001 high throughput mutant phenotype evidence used in manual assertion

Back to: Guide to GO Evidence Codes