https://wiki.geneontology.org/index.php?title=LEGO_June_1,_2015&feed=atom&action=historyLEGO June 1, 2015 - Revision history2024-03-29T08:49:12ZRevision history for this page on the wikiMediaWiki 1.40.0https://wiki.geneontology.org/index.php?title=LEGO_June_1,_2015&diff=57226&oldid=prevVanaukenk: /* Annotation Issues */2015-05-29T15:47:14Z<p><span dir="auto"><span class="autocomment">Annotation Issues</span></span></p>
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 11:47, 29 May 2015</td>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>***We further showed that GFP::moesinABD was largely reduced at the leading edge (Figures 3B, 3D, and 3E): the fluorescence intensity ratio between the green F-actin and the red plasma membrane was decreased from 4.2- to 0.3-fold during Q cell migration and from 4.0- to 0.2-fold during neurite growth in ani-1 conditional knockouts (Figures 3F and S3). Thus, Anillin regulates neuronal migration and neurite growth by stabilizing the actin cytoskeleton in the leading edge.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>***We further showed that GFP::moesinABD was largely reduced at the leading edge (Figures 3B, 3D, and 3E): the fluorescence intensity ratio between the green F-actin and the red plasma membrane was decreased from 4.2- to 0.3-fold during Q cell migration and from 4.0- to 0.2-fold during neurite growth in ani-1 conditional knockouts (Figures 3F and S3). Thus, Anillin regulates neuronal migration and neurite growth by stabilizing the actin cytoskeleton in the leading edge.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>***Under a low centrifugation force (see the Supplemental Experimental Procedures), we showed that the presence of Anillin or its MBD-ABD domain increased the amount of preassembled actin filaments in the pellet(Figure4E;Plastin 3 as the positive control), suggesting that Anillin may organize actin filaments into higher-order structures. This bundling activity was further confirmed by directly visualizing the effects of these Anillin constructs on preassembled actin filaments under a fluorescence microscope (Figure 4F).</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>***Under a low centrifugation force (see the Supplemental Experimental Procedures), we showed that the presence of Anillin or its MBD-ABD domain increased the amount of preassembled actin filaments in the pellet(Figure4E;Plastin 3 as the positive control), suggesting that Anillin may organize actin filaments into higher-order structures. This bundling activity was further confirmed by directly visualizing the effects of these Anillin constructs on preassembled actin filaments under a fluorescence microscope (Figure 4F).</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>**positively_regulates</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>**positively_regulates <ins style="font-weight: bold; text-decoration: none;">: x positively regulates y if and only if the progression of x increases the frequency, rate or extent of y</ins></div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>***We showed that 20% of A/PQR neurons reduced their migration distance in ani-1 conditional mutants (Figures 2C and 2E).</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>***We showed that 20% of A/PQR neurons reduced their migration distance in ani-1 conditional mutants (Figures 2C and 2E).</div></td></tr>
</table>Vanaukenkhttps://wiki.geneontology.org/index.php?title=LEGO_June_1,_2015&diff=57225&oldid=prevVanaukenk: /* Annotation Issues */2015-05-29T15:28:52Z<p><span dir="auto"><span class="autocomment">Annotation Issues</span></span></p>
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">← Older revision</td>
<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 11:28, 29 May 2015</td>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>***Under a low centrifugation force (see the Supplemental Experimental Procedures), we showed that the presence of Anillin or its MBD-ABD domain increased the amount of preassembled actin filaments in the pellet(Figure4E;Plastin 3 as the positive control), suggesting that Anillin may organize actin filaments into higher-order structures. This bundling activity was further confirmed by directly visualizing the effects of these Anillin constructs on preassembled actin filaments under a fluorescence microscope (Figure 4F).</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>***Under a low centrifugation force (see the Supplemental Experimental Procedures), we showed that the presence of Anillin or its MBD-ABD domain increased the amount of preassembled actin filaments in the pellet(Figure4E;Plastin 3 as the positive control), suggesting that Anillin may organize actin filaments into higher-order structures. This bundling activity was further confirmed by directly visualizing the effects of these Anillin constructs on preassembled actin filaments under a fluorescence microscope (Figure 4F).</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>**positively_regulates</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>**positively_regulates</div></td></tr>
<tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">***We showed that 20% of A/PQR neurons reduced their migration distance in ani-1 conditional mutants (Figures 2C and 2E).</ins></div></td></tr>
</table>Vanaukenkhttps://wiki.geneontology.org/index.php?title=LEGO_June_1,_2015&diff=57224&oldid=prevVanaukenk: /* Annotation Issues */2015-05-29T15:27:21Z<p><span dir="auto"><span class="autocomment">Annotation Issues</span></span></p>
<table style="background-color: #fff; color: #202122;" data-mw="interface">
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">← Older revision</td>
<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 11:27, 29 May 2015</td>
</tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l26">Line 26:</td>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>***To test this, we introduced GFP::ANI-1 into mig-2(mu28)-null mutants, and we showed that GFP::ANI-1 left the plasma membrane and became evenly distributed in the cytoplasm in mig-2(mu28) animals (Figures 6 A–6E for QR.ap;Movie S2).</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>***To test this, we introduced GFP::ANI-1 into mig-2(mu28)-null mutants, and we showed that GFP::ANI-1 left the plasma membrane and became evenly distributed in the cytoplasm in mig-2(mu28) animals (Figures 6 A–6E for QR.ap;Movie S2).</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>***We further showed that GFP::moesinABD was largely reduced at the leading edge (Figures 3B, 3D, and 3E): the fluorescence intensity ratio between the green F-actin and the red plasma membrane was decreased from 4.2- to 0.3-fold during Q cell migration and from 4.0- to 0.2-fold during neurite growth in ani-1 conditional knockouts (Figures 3F and S3). Thus, Anillin regulates neuronal migration and neurite growth by stabilizing the actin cytoskeleton in the leading edge.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>***We further showed that GFP::moesinABD was largely reduced at the leading edge (Figures 3B, 3D, and 3E): the fluorescence intensity ratio between the green F-actin and the red plasma membrane was decreased from 4.2- to 0.3-fold during Q cell migration and from 4.0- to 0.2-fold during neurite growth in ani-1 conditional knockouts (Figures 3F and S3). Thus, Anillin regulates neuronal migration and neurite growth by stabilizing the actin cytoskeleton in the leading edge.</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>***Under a low centrifugation force (see the Supplemental Experimental Procedures), we showed that the presence of Anillin or its MBD-ABD domain increased the amount of preassembled actin filaments in the pellet(Figure4E;Plastin 3 as the positive control), suggesting that Anillin may organize actin filaments into higher-order structures. This bundling activity was further confirmed</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>***Under a low centrifugation force (see the Supplemental Experimental Procedures), we showed that the presence of Anillin or its MBD-ABD domain increased the amount of preassembled actin filaments in the pellet(Figure4E;Plastin 3 as the positive control), suggesting that Anillin may organize actin filaments into higher-order structures. This bundling activity was further confirmed by directly visualizing the effects of these Anillin constructs on preassembled actin filaments under a fluorescence microscope (Figure 4F).</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>by directly visualizing the effects of these Anillin constructs on preassembled actin filaments under a fluorescence microscope (Figure 4F).</div></td><td colspan="2" class="diff-side-added"></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>**positively_regulates</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>**positively_regulates</div></td></tr>
</table>Vanaukenkhttps://wiki.geneontology.org/index.php?title=LEGO_June_1,_2015&diff=57223&oldid=prevVanaukenk: /* Annotation Issues */2015-05-29T15:26:45Z<p><span dir="auto"><span class="autocomment">Annotation Issues</span></span></p>
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">← Older revision</td>
<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 11:26, 29 May 2015</td>
</tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l26">Line 26:</td>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>***To test this, we introduced GFP::ANI-1 into mig-2(mu28)-null mutants, and we showed that GFP::ANI-1 left the plasma membrane and became evenly distributed in the cytoplasm in mig-2(mu28) animals (Figures 6 A–6E for QR.ap;Movie S2).</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>***To test this, we introduced GFP::ANI-1 into mig-2(mu28)-null mutants, and we showed that GFP::ANI-1 left the plasma membrane and became evenly distributed in the cytoplasm in mig-2(mu28) animals (Figures 6 A–6E for QR.ap;Movie S2).</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>***We further showed that GFP::moesinABD was largely reduced at the leading edge (Figures 3B, 3D, and 3E): the fluorescence intensity ratio between the green F-actin and the red plasma membrane was decreased from 4.2- to 0.3-fold during Q cell migration and from 4.0- to 0.2-fold during neurite growth in ani-1 conditional knockouts (Figures 3F and S3). Thus, Anillin regulates neuronal migration and neurite growth by stabilizing the actin cytoskeleton in the leading edge.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>***We further showed that GFP::moesinABD was largely reduced at the leading edge (Figures 3B, 3D, and 3E): the fluorescence intensity ratio between the green F-actin and the red plasma membrane was decreased from 4.2- to 0.3-fold during Q cell migration and from 4.0- to 0.2-fold during neurite growth in ani-1 conditional knockouts (Figures 3F and S3). Thus, Anillin regulates neuronal migration and neurite growth by stabilizing the actin cytoskeleton in the leading edge.</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>***Under a low centrifugation force (see the Supplemental Experimental Procedures), we showed that the presence of Anillin or its</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>***Under a low centrifugation force (see the Supplemental Experimental Procedures), we showed that the presence of Anillin or its MBD-ABD domain increased the amount of preassembled actin filaments in the pellet(Figure4E;Plastin 3 as the positive control), suggesting that Anillin may organize actin filaments into higher-order structures. This bundling activity was further confirmed</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>MBD-ABD domain increased the amount of preassembled actin filaments in the pellet(Figure4E;Plastin 3 as the positive control), suggesting that Anillin may organize actin filaments into higher-order structures. This bundling activity was further confirmed</div></td><td colspan="2" class="diff-side-added"></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>by directly visualizing the effects of these Anillin constructs on preassembled actin filaments under a fluorescence microscope (Figure 4F).</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>by directly visualizing the effects of these Anillin constructs on preassembled actin filaments under a fluorescence microscope (Figure 4F).</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>**positively_regulates</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>**positively_regulates</div></td></tr>
</table>Vanaukenkhttps://wiki.geneontology.org/index.php?title=LEGO_June_1,_2015&diff=57222&oldid=prevVanaukenk: /* Annotation Issues */2015-05-29T15:26:17Z<p><span dir="auto"><span class="autocomment">Annotation Issues</span></span></p>
<table style="background-color: #fff; color: #202122;" data-mw="interface">
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">← Older revision</td>
<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 11:26, 29 May 2015</td>
</tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l26">Line 26:</td>
<td colspan="2" class="diff-lineno">Line 26:</td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>***To test this, we introduced GFP::ANI-1 into mig-2(mu28)-null mutants, and we showed that GFP::ANI-1 left the plasma membrane and became evenly distributed in the cytoplasm in mig-2(mu28) animals (Figures 6 A–6E for QR.ap;Movie S2).</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>***To test this, we introduced GFP::ANI-1 into mig-2(mu28)-null mutants, and we showed that GFP::ANI-1 left the plasma membrane and became evenly distributed in the cytoplasm in mig-2(mu28) animals (Figures 6 A–6E for QR.ap;Movie S2).</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>***We further showed that GFP::moesinABD was largely reduced at the leading edge (Figures 3B, 3D, and 3E): the fluorescence intensity ratio between the green F-actin and the red plasma membrane was decreased from 4.2- to 0.3-fold during Q cell migration and from 4.0- to 0.2-fold during neurite growth in ani-1 conditional knockouts (Figures 3F and S3). Thus, Anillin regulates neuronal migration and neurite growth by stabilizing the actin cytoskeleton in the leading edge.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>***We further showed that GFP::moesinABD was largely reduced at the leading edge (Figures 3B, 3D, and 3E): the fluorescence intensity ratio between the green F-actin and the red plasma membrane was decreased from 4.2- to 0.3-fold during Q cell migration and from 4.0- to 0.2-fold during neurite growth in ani-1 conditional knockouts (Figures 3F and S3). Thus, Anillin regulates neuronal migration and neurite growth by stabilizing the actin cytoskeleton in the leading edge.</div></td></tr>
<tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">***Under a low centrifugation force (see the Supplemental Experimental Procedures), we showed that the presence of Anillin or its</ins></div></td></tr>
<tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">MBD-ABD domain increased the amount of preassembled actin filaments in the pellet(Figure4E;Plastin 3 as the positive control), suggesting that Anillin may organize actin filaments into higher-order structures. This bundling activity was further confirmed</ins></div></td></tr>
<tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">by directly visualizing the effects of these Anillin constructs on preassembled actin filaments under a fluorescence microscope (Figure 4F).</ins></div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>**positively_regulates</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>**positively_regulates</div></td></tr>
</table>Vanaukenkhttps://wiki.geneontology.org/index.php?title=LEGO_June_1,_2015&diff=57221&oldid=prevVanaukenk: /* Annotation Issues */2015-05-29T15:21:26Z<p><span dir="auto"><span class="autocomment">Annotation Issues</span></span></p>
<table style="background-color: #fff; color: #202122;" data-mw="interface">
<col class="diff-marker" />
<col class="diff-content" />
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">← Older revision</td>
<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 11:21, 29 May 2015</td>
</tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l25">Line 25:</td>
<td colspan="2" class="diff-lineno">Line 25:</td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>**directly_activates : p directly activates q if and only if p is immediately upstream of q and p is the realization of a function to increase the rate or activity of q</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>**directly_activates : p directly activates q if and only if p is immediately upstream of q and p is the realization of a function to increase the rate or activity of q</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>***To test this, we introduced GFP::ANI-1 into mig-2(mu28)-null mutants, and we showed that GFP::ANI-1 left the plasma membrane and became evenly distributed in the cytoplasm in mig-2(mu28) animals (Figures 6 A–6E for QR.ap;Movie S2).</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>***To test this, we introduced GFP::ANI-1 into mig-2(mu28)-null mutants, and we showed that GFP::ANI-1 left the plasma membrane and became evenly distributed in the cytoplasm in mig-2(mu28) animals (Figures 6 A–6E for QR.ap;Movie S2).</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>***</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>***<ins style="font-weight: bold; text-decoration: none;">We further showed that GFP::moesinABD was largely reduced at the leading edge (Figures 3B, 3D, and 3E): the fluorescence intensity ratio between the green F-actin and the red plasma membrane was decreased from 4.2- to 0.3-fold during Q cell migration and from 4.0- to 0.2-fold during neurite growth in ani-1 conditional knockouts (Figures 3F and S3). Thus, Anillin regulates neuronal migration and neurite growth by stabilizing the actin cytoskeleton in the leading edge.</ins></div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>**positively_regulates</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>**positively_regulates</div></td></tr>
</table>Vanaukenkhttps://wiki.geneontology.org/index.php?title=LEGO_June_1,_2015&diff=57220&oldid=prevVanaukenk: /* Annotation Issues */2015-05-29T15:18:41Z<p><span dir="auto"><span class="autocomment">Annotation Issues</span></span></p>
<table style="background-color: #fff; color: #202122;" data-mw="interface">
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<col class="diff-content" />
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">← Older revision</td>
<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 11:18, 29 May 2015</td>
</tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l23">Line 23:</td>
<td colspan="2" class="diff-lineno">Line 23:</td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>**causally_upstream_of : p is upstream of q if and only if p precedes q and p and q are linked in a causal chain</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>**causally_upstream_of : p is upstream of q if and only if p precedes q and p and q are linked in a causal chain</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>***To test this, we introduced GFP::ANI-1 into mig-2(mu28)-null mutants, and we showed that GFP::ANI-1 left the plasma membrane and became evenly distributed in the cytoplasm in mig-2(mu28) animals (Figures 6 A–6E for QR.ap;Movie S2).</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>***To test this, we introduced GFP::ANI-1 into mig-2(mu28)-null mutants, and we showed that GFP::ANI-1 left the plasma membrane and became evenly distributed in the cytoplasm in mig-2(mu28) animals (Figures 6 A–6E for QR.ap;Movie S2).</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>**directly_activates</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>**directly_activates <ins style="font-weight: bold; text-decoration: none;">: p directly activates q if and only if p is immediately upstream of q and p is the realization of a function to increase the rate or activity of q</ins></div></td></tr>
<tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">***To test this, we introduced GFP::ANI-1 into mig-2(mu28)-null mutants, and we showed that GFP::ANI-1 left the plasma membrane and became evenly distributed in the cytoplasm in mig-2(mu28) animals (Figures 6 A–6E for QR.ap;Movie S2).</ins></div></td></tr>
<tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">***</ins></div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>**positively_regulates</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>**positively_regulates</div></td></tr>
</table>Vanaukenkhttps://wiki.geneontology.org/index.php?title=LEGO_June_1,_2015&diff=57219&oldid=prevVanaukenk: /* Annotation Issues */2015-05-29T15:16:02Z<p><span dir="auto"><span class="autocomment">Annotation Issues</span></span></p>
<table style="background-color: #fff; color: #202122;" data-mw="interface">
<col class="diff-marker" />
<col class="diff-content" />
<col class="diff-marker" />
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<tr class="diff-title" lang="en">
<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">← Older revision</td>
<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 11:16, 29 May 2015</td>
</tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l20">Line 20:</td>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>**ANI-1: GTP-Rho binding? Rho GTPase binding?</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>**ANI-1: GTP-Rho binding? Rho GTPase binding?</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>*What's the correct MF annotation for MIG-2 for this signaling pathway?</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>*What's the correct MF annotation for MIG-2 for this signaling pathway?</div></td></tr>
<tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">*Wrt linking annotons, clarify what experimental evidence supports use of:</ins></div></td></tr>
<tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">**causally_upstream_of : p is upstream of q if and only if p precedes q and p and q are linked in a causal chain</ins></div></td></tr>
<tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">***To test this, we introduced GFP::ANI-1 into mig-2(mu28)-null mutants, and we showed that GFP::ANI-1 left the plasma membrane and became evenly distributed in the cytoplasm in mig-2(mu28) animals (Figures 6 A–6E for QR.ap;Movie S2).</ins></div></td></tr>
<tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">**directly_activates</ins></div></td></tr>
<tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">**positively_regulates</ins></div></td></tr>
</table>Vanaukenkhttps://wiki.geneontology.org/index.php?title=LEGO_June_1,_2015&diff=57218&oldid=prevVanaukenk: /* GTPase Signaling Pathways */2015-05-29T15:14:15Z<p><span dir="auto"><span class="autocomment">GTPase Signaling Pathways</span></span></p>
<table style="background-color: #fff; color: #202122;" data-mw="interface">
<col class="diff-marker" />
<col class="diff-content" />
<col class="diff-marker" />
<col class="diff-content" />
<tr class="diff-title" lang="en">
<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">← Older revision</td>
<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 11:14, 29 May 2015</td>
</tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l10">Line 10:</td>
<td colspan="2" class="diff-lineno">Line 10:</td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>**We have a term, GO:0030742, GTP-dependent protein binding; what is the intended use of this term?</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>**We have a term, GO:0030742, GTP-dependent protein binding; what is the intended use of this term?</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>***Definition: Interacting selectively and non-covalently with any protein or protein complex (a complex of two or more proteins that may include other nonprotein molecules) using energy from the hydrolysis of GTP.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>***Definition: Interacting selectively and non-covalently with any protein or protein complex (a complex of two or more proteins that may include other nonprotein molecules) using energy from the hydrolysis of GTP.</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">*Wrt linking annotons, clarify what experimental evidence supports use of:</del></div></td><td colspan="2" class="diff-side-added"></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">**causally_upstream_of : p is upstream of q if and only if p precedes q and p and q are linked in a causal chain</del></div></td><td colspan="2" class="diff-side-added"></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">***To test this, we introduced GFP::ANI-1 into mig-2(mu28)-null mutants, and we showed that GFP::ANI-1 left the plasma membrane and became evenly distributed in the cytoplasm in mig-2(mu28) animals (Figures 6 A–6E for QR.ap;Movie S2).</del></div></td><td colspan="2" class="diff-side-added"></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">**directly_activates</del></div></td><td colspan="2" class="diff-side-added"></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">**positively_regulates</del></div></td><td colspan="2" class="diff-side-added"></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>===Annotation Issues===</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>===Annotation Issues===</div></td></tr>
</table>Vanaukenkhttps://wiki.geneontology.org/index.php?title=LEGO_June_1,_2015&diff=57217&oldid=prevVanaukenk: /* GTPase Signaling Pathways */2015-05-29T15:13:13Z<p><span dir="auto"><span class="autocomment">GTPase Signaling Pathways</span></span></p>
<table style="background-color: #fff; color: #202122;" data-mw="interface">
<col class="diff-marker" />
<col class="diff-content" />
<col class="diff-marker" />
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<tr class="diff-title" lang="en">
<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">← Older revision</td>
<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 11:13, 29 May 2015</td>
</tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l11">Line 11:</td>
<td colspan="2" class="diff-lineno">Line 11:</td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>***Definition: Interacting selectively and non-covalently with any protein or protein complex (a complex of two or more proteins that may include other nonprotein molecules) using energy from the hydrolysis of GTP.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>***Definition: Interacting selectively and non-covalently with any protein or protein complex (a complex of two or more proteins that may include other nonprotein molecules) using energy from the hydrolysis of GTP.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>*Wrt linking annotons, clarify what experimental evidence supports use of:</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>*Wrt linking annotons, clarify what experimental evidence supports use of:</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>**causally_upstream_of</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>**causally_upstream_of <ins style="font-weight: bold; text-decoration: none;">: p is upstream of q if and only if p precedes q and p and q are linked in a causal chain</ins></div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>***To test this, we introduced GFP::ANI-1 into mig-2(mu28)-null mutants, and we showed that GFP::ANI-1 left the plasma membrane and became evenly distributed in the cytoplasm in mig-2(mu28) animals (Figures 6 A–6E for QR.ap;Movie S2).</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>***To test this, we introduced GFP::ANI-1 into mig-2(mu28)-null mutants, and we showed that GFP::ANI-1 left the plasma membrane and became evenly distributed in the cytoplasm in mig-2(mu28) animals (Figures 6 A–6E for QR.ap;Movie S2).</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>**directly_activates</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>**directly_activates</div></td></tr>
</table>Vanaukenk