MGI, March 2010
Mouse Genome Informatics March, 2010
Overview
Staff:
Judith Blake
Alexander Diehl
Harold J Drabkin
David Hill
Li Ni
Dmitry Sitnikov
Mary Dolan
Annotation Progress
We continue to put emphasis on those genes selected for the Reference Genome Project. Additional emphasis has been placed on certain genes associated with lung development.
Annotation Type | 01_Sept_09 | 22_Mar_10 | Change | % Change |
Total Genes annotated (with at least one GO term of any kind): | 18188
|
35191*
|
16993
|
93.43
|
Total Manual Annotation | ||||
Number of Genes | 11177
|
33228
|
22051
|
197.29
|
Orthology: | 708
|
4006
|
3298
|
465.82
|
IEA Annotation | ||||
SwissProt to GO | 16145
|
15942
|
62
|
0.39
|
Interpro to GO | 10533
|
10592
|
-3
|
-0.03
|
EC to GO | 1491
|
1248
|
13
|
0.89
|
* 100% of current gene models |
Methods and strategies for annotation
Literature curation:
Literature curation continues to be the major focus of our annotation efforts. Wecontinue to explore natural language processing tools to aid in identifying papers that are primarily focused on aspects of lung development, with the aid of Karen Dowel.
Computational annotation strategies:
As always current strategies involve use of translation table to mine SwissProt Keywords and InterPro domains for IEA annotation. These are performed automatically on a nightly basis and require little human intervention.
Priorities for annotation
- Genes assigned by Reference Genome Project (everyone)
- Isoform curation (Harold, Protein Ontology project); now co-ordinating with 1 by focusing on reference genes that have isoforms.
- Genes with no GO annotation but with literature (Li and Dmitry)
- Genes identified as being important in lung development (Dmitry)
- Genes marked as having GO annotation completed, but now having new literature (Dmitry)
Presentations and Publications
b. Presentations including Talks and Tutorials and Teaching
A. Ontology Development Contributions:
1. David Hill has worked on a team with Tanya Berardini, Chris Mungall, Midori Harris, Jen Deegan and Jane Lomax to develop cross-products within the three GO namespaces. The regulation cross-products have been released in the extended GO. David and Tanya are now quality checking the internal biological process cross products.
2. David Hill has worked Tanya Berardini to continuing to add interontology links between MF and BP.
3. David Hill has worked with Varsha Khodiyar, Tanya Berardini, Doug Howe, Susan Tweedie, Ruth Lovering and community experts to expand the heart development portionof the ontology.
4. David Hill has worked with Yasmin Alam-Faruque, Midori Harris, Becky Folger, Doug Howe, Emily Dimmer, Rachel Huntley and community experts to expand the kidney development portion of the ontology.
5. David Hill and Tanya Berardini are maintaining the automated quality checks of the ontology on an ongoing basis.
6. David Hill and Harold Drabkin are working with Jane Lomax, Midori Harris, Tanya Berardini and Jane Lomax to align the representation of chemicals in GO.
7. David Hill and Tanya Berardini added or modified 231 terms as a result of attending the American Society for Cell Biology Meeting in December.
Annotation Outreach and User Advocacy Efforts:
The Protein Ontology project is providing a web interface (http/pir.georgetown.edu/cgi-bin/pro/race_pro ) whereby functional annotation using the GO can be applied to PRO submissions. These are reviewed by Cecilia Arighi of Georgetown. At present, only the PRO curators (Georgetown and MGI) are using the tool, but it is available to anyone.
Harold and David are mentoring Heather Wick of Tufts University, for annotation of human fetal lung development
Other Highlights:
We are now suppling a GAF 2.0 format file to GOC with column 16 and 17 data filled in. This file is also available directly from our own FTP site. Currently, the data in column 16 refers to the cell type that the experiment supporting the annotation was carried out in, by use of cell ontology terms.