MGI December 2012

From GO Wiki
Revision as of 09:23, 10 December 2012 by David (talk | contribs) (Annotation Progress)

Jump to: navigation, search

Mouse Genome Informatics Summary, December 2011

Overview

Staff:

Judith Blake*

Karen R Christie*

Mary Dolan*

Harold J Drabkin*

David Hill*

Li Ni

Dmitry Sitnikov

* Funded entirely or partially by GO

Annotation Progress

MGI GO STATS as of December 2012

This year our manual annotation effort has focused on obtaining annotations for genes that had no annotations or on obtaining deeper manual annotations to terms that were annotated with IEA or one of the root nodes of GO.

Annotation Type
05_Dec_2011
05_Dec_2012
Change
% Change
Total Genes annotated with at least one GO term of any kind
25109
25452
343
1.4
Total annotations:
272241
286957
14716
5.4
Total non-IEA Annotation
 
Total Number of Genes
24029
24550
521
2.2
Total Annotations
178496
193088
14592
8.2
Annotation by Direct Experiment
 
 
 
 
MGI Curated Genes
10332
11104
772
7.5
MGI Curated Annotations
64165
70615
6450
10.1
GOA Curated Genes
2910
3475
565
19.4
GOA Curated Annotations
14048
18055
4007
28.5
Annotation by Orthology
 
 
 
 
Genes Annotated by Orthology Total
8912
9451
539
6.0
Total Orthology Annotations
62606
68621
6015
9.6
Genes Annotated by Human Orthology Load (GOA)
7171
7756
585
8.2
Total Annotation by Human Orthology Load
36796
42237
5441
14.8
Genes annotated by Rat Orthology Load (RGD)
3963
4083
120
3.0
Total Annotations by Rat Orthology Load
22390
23323
933
4.2
IEA Annotation
 
 
 
 
Total Genes with IEA
16319
16372
53
0.3
Total IEA annotations
93745
93869
124
0.1
Total Genes with SwissProt to GO
15803
15971
168
1.1
Total SwissProt to GO Annotations
62283
64733
2450
3.9
Total Genes with Interpro to GO
9781
9792
11
0.1
Total Interpro to GO Annotations
29954
27804
-2150
-7.2
Total Genes with EC to GO Annotations
930
944
14
1.5
Total EC to GO annotations
1508
1332
-176
-11.7



Methods and strategies for annotation

Literature curation:

Literature curation continues to be the major focus of our annotation efforts.

Computational annotation strategies:

As always, current strategies involve use of translation table to mine SwissProt keywords, InterPro domains, and EC numbers for IEA annotation. These are performed automatically on a nightly basis and require little human intervention.

Priorities for annotation

  • Isoform curation (Harold, Protein Ontology project); now co-ordinating with 1 by focusing on reference genes that have isoforms, and co-ordinated with the Protein Ontology Protein Complex project.
  • Genes with no GO annotation but with literature (Li and Dmitry)
  • Genes with only IEA annotation but with literature (Li)
  • Genes identified as being important in lung development (Dmitry)
  • Genes marked as having GO annotation completed, but now having new literature (Dmitry)
  • Genes that have an annotation to one of the three root nodes of GO, but have new literature (David, Karen, Dmitry)
  • Dmitry has been focused on annotation or miRNAs in MGI

Presentations and Publications

Drabkin HJ, Blake JA; for the Mouse Genome Informatics Database. Manual Gene Ontology annotation workflow at the Mouse Genome Informatics Database. Database (Oxford). 2012 Oct 29;2012(0):bas045. Print 2012. PubMed PMID: 23110975; PubMed Central PMCID: PMC3483533.


Taşan M, Drabkin HJ, Beaver JE, Chua HN, Dunham J, Tian W, Blake JA, Roth FP. A Resource of Quantitative Functional Annotation for Homo sapiens Genes. G3 (Bethesda). 2012 Feb;2(2):223-33. Epub 2012 Feb 1. PubMed PMID: 22384401; PubMed Central PMCID: PMC3284330.

Ontology Development Contributions:

  • 1. David Hill, working with Tanya Berardini, Chris Mungall, Midori Harris and Jane Lomax develop cross-products within and among the three GO namespaces.
  • 2. David Hill works with Chris Mungall and Tanya Berardini to add inter-ontology links in GO.
  • 3. David Hill and Jane Lomax oversee the biological content development of GO. In particular, all new developmental biology-related terms submitted to SourceForge are handled by David Hill.
  • 4. David Hill and Harold Drabkin continue working with Tanya Berardini, Chris Mungall, Midori Harris, Jane Lomax and ChEBI curators to align GO with ChEBI. This will result in the first set of cross-products with GO and an external ontology.
  • 5. David Hill and Karen Christie continue to revise and update the transcription area of GO.
  • 6. Harold Drabkin is working on improvement of the the representation of tRNA modification

Annotation Outreach and User Advocacy Efforts:

  • The Protein Ontology project continues to provide a web interface (http/pir.georgetown.edu/cgi-bin/pro/race_pro) whereby functional annotation using the GO can be applied to PRO submissions. Harold attended the Protein Society meeting in Boston to try to encourage bench scientists to use the too.
  • David Hill and Harold Drabkin serve on the GO-help rota.
  • Terry Meehan, Chris Mungall, and Alex Diehl (Univ. of Buffalo) continue their editing of the cell ontology (CL) including cross-products to the GO. Chris is helping David Hill in 'mining 'notes in MGI's GO annotations that reference to CL identifiers.

Other Highlights:

  • As the designated coordinator of the MGI/GO project with the GO Reference Genome project, Li Ni participates in annotations of genes assigned by the Reference Genome Project, maintain the mouse Reference Genome list on MGI GO wiki and Google spreadsheet, maintain the Reference Genome status table on GO wiki, oversees the curation of Reference Genome Genes for the mouse group. Li responds and resolves questions about MGI GO annotations for the reference genome annotation project genes, and especially responds and resolves questions from the lead PAINT curator (see Reference Genome Project report for a description of PAINT).
  • Mary Dolan has been involved in a collaboration with Carol Bult at MGI on aligning gene ontology annotations for mouse genes assigned to MouseCyc pathways (See http://www.informatics.jax.org/pathways.shtml) and exploring computational methods for associating functional, pathway, and phenotypic data. Mary also provides various files for the Reference Genome Project, for example, a report to assess the GO annotation status of PANTHER families and subfamilies based on annotations for all reference genome organism genes in the groups.