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| Here are the steps that I can think of in doing manual literature-based annotation:
| | [[Category:Annotation Archived]] |
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| | | [[Image:literature_original-4thMay.ppt]] |
| 1) Find a paper that shows the function of a gene product or the biological process or cellular location in which it acts.
| | [[Image:literature_original-4thMay.png]] |
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| 2) Find the accession number of the gene product, e.g. from UniProt or GenBank.
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| 3) Find the GO term that is appropriate using AmiGO (e.g. GO:0048256).
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| 4) Choose the evidence code for the experiment that was done (e.g. IDA). <br>(See http://www.geneontology.org/GO.evidence.shtml and
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| http://www.geneontology.org/GO.evidence.codes.shtml)
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| 5) Note down the pubmed id for the paper. (e.g. PMID: 17277778)<br>(See http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed)
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| 6) Put these in a spreadsheet with the appropriate headings.
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| Or put them in a tab-delimited file.<br> (See http://www.geneontology.org/GO.annotation.shtml#fields)
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| 7) Send to the GO Consortium.
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| Here is my first draft powerpoint image
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| [[Image:Literature_flowchart.png]] | |
| [[Image:literature.ppt]]
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| wondered if you would find it useful..Evelyn
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| [[Image:manualannot.jpg]] | |
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Latest revision as of 11:35, 14 March 2019
