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| Here are the steps that I can think of in doing manual literature-based annotation:
| | [[Category:Annotation Archived]] |
| | Current latest: |
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| | | [[Image:literature_original-4thMay.ppt]] |
| Find a paper that shows the function of a gene product or the biological process or cellular location in which it acts.<br>
| | [[Image:literature_original-4thMay.png]] |
| http://www.ncbi.nlm.nih.gov/entrez/<br>
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| (Should look like PMID:XXXXXXXX.)<br>
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| Find the accession number of the gene product, e.g. from UniProt, DDBJ or GenBank.<br>
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| http://www.ebi.uniprot.org/index.shtml<br>
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| http://www.ncbi.nlm.nih.gov/<br>
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| http://www.ddbj.nig.ac.jp/<br>
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| Find the GO term that is appropriate using AmiGO<br>
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| http://amigo.geneontology.org/<br>
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| (Should look like GO:XXXXXXX.)<br>
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| Choose the evidence code for the experiment that was done (e.g. IDA). <br>
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| (See http://www.geneontology.org/GO.evidence.shtml and http://www.geneontology.org/GO.evidence.codes.shtml)<br>
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| Write down the taxon id of the organism from which your gene product is derived. <br>
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| http://www.ncbi.nlm.nih.gov/Taxonomy/<br>
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| (Should look like Taxonomy ID: XXXX)<br>
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| Put these in a spreadsheet or tab-delimited file with the appropriate headings. <br>
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| [[Image:Literature.png]]
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| [[Image:literature_original.ppt]] | |
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| Pascale's improved version:
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| [[Image:literature_original-PG.ppt]] | |
| [[Image:Picture 2.png]]
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| wondered if you would find it useful..Evelyn
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| [[Image:manualannot.jpg]]
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