|
|
(5 intermediate revisions by 3 users not shown) |
Line 1: |
Line 1: |
| Here are the steps that I can think of in doing manual literature-based annotation:
| | [[Category:Annotation Archived]] |
| | Current latest: |
|
| |
|
| | | [[Image:literature_original-4thMay.ppt]] |
| Find a paper that shows the function of a gene product or the biological process or cellular location in which it acts.<br>
| | [[Image:literature_original-4thMay.png]] |
| http://www.ncbi.nlm.nih.gov/entrez/<br>
| |
| (Should look like PMID:XXXXXXXX.)<br>
| |
| | |
| | |
| Find the accession number of the gene product, e.g. from UniProt, DDBJ or GenBank.<br>
| |
| http://www.ebi.uniprot.org/index.shtml<br>
| |
| http://www.ncbi.nlm.nih.gov/<br>
| |
| http://www.ddbj.nig.ac.jp/<br>
| |
| | |
| | |
| Find the GO term that is appropriate using AmiGO<br>
| |
| http://amigo.geneontology.org/<br>
| |
| (Should look like GO:XXXXXXX.)<br>
| |
| | |
| | |
| Choose the evidence code for the experiment that was done (e.g. IDA). <br>
| |
| (See http://www.geneontology.org/GO.evidence.shtml and http://www.geneontology.org/GO.evidence.codes.shtml)<br>
| |
| | |
| | |
| Write down the taxon id of the organism from which your gene product is derived. <br>
| |
| http://www.ncbi.nlm.nih.gov/Taxonomy/<br>
| |
| (Should look like Taxonomy ID: XXXX)<br>
| |
| | |
| | |
| Put these in a spreadsheet or tab-delimited file with the appropriate headings. <br>
| |
| | |
| | |
| [[Image:Literature.png]]
| |
| | |
| | |
| | |
| [[Image:literature_original.ppt]]
| |
| | |
| | |
| | |
| Pascale's improved version:
| |
| | |
| [[Image:literature_original-PG.ppt]] | |
| [[Image:literature_original-PG.png]] | |
| | |
| wondered if you would find it useful..Evelyn
| |
| [[Image:manualannot.jpg]]
| |
| ---------------------------------
| |