Manual - Jennifer (Archived): Difference between revisions

Revision as of 09:26, 6 February 2007

Here are the steps that I can think of in doing manual annotation:

1) Find a paper that shows the function of a gene product or the biological process or cellular location in which it acts.

2) Find the accession number of the gene product, e.g. from UniProt or GenBank.

3) Find the GO term that is appropriate using AmiGO (e.g. GO:0048256).

5) Note down the pubmed id for the paper. (e.g. PMID: 17277778)
(See http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed)

6) Put these in a spreadsheet with the appropriate headings. Or put them in a tab-delimited file.
(See http://www.geneontology.org/GO.annotation.shtml#fields)

7) Send to the GO Consortium.

Q) Shall we also include all the other fields or will this get very complicated?

@@ Line 6: / Line 6: @@
 ) Find the accession number of the gene product, e.g. from UniProt or GenBank.
-) Find the GO term that is appropriate using AmiGO.
+) Find the GO term that is appropriate using AmiGO (e.g. GO:0048256).
-) Choose the evidence code for the experiment that was done. (See evidence code docs.)
+) Choose the evidence code for the experiment that was done (e.g. IDA). <br>(See http://www.geneontology.org/GO.evidence.shtml and
+http://www.geneontology.org/GO.evidence.codes.shtml)
-) Note down the pubmed id for the paper.
+) Note down the pubmed id for the paper.  (e.g. PMID: 17277778)<br>(See http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed)
 ) Put these in a spreadsheet with the appropriate headings.
-(Shall we provide a spreadsheet with the headings?
+Or put them in a tab-delimited file.<br> (See http://www.geneontology.org/GO.annotation.shtml#fields)
 ) Send to the GO Consortium.