Manual - Jennifer (Archived)

Here are the steps that I can think of in doing manual literature-based annotation:

1) Find a paper that shows the function of a gene product or the biological process or cellular location in which it acts.

2) Find the accession number of the gene product, e.g. from UniProt or GenBank.

3) Find the GO term that is appropriate using AmiGO (e.g. GO:0048256).

4) Choose the evidence code for the experiment that was done (e.g. IDA).
(See http://www.geneontology.org/GO.evidence.shtml and http://www.geneontology.org/GO.evidence.codes.shtml)

5) Note down the pubmed id for the paper. (e.g. PMID: 17277778)
(See http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed)

6) Put these in a spreadsheet with the appropriate headings. Or put them in a tab-delimited file.
(See http://www.geneontology.org/GO.annotation.shtml#fields)

7) Send to the GO Consortium.

Here is my first draft powerpoint image

File:Literature.ppt

wondered if you would find it useful..Evelyn

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