Panther ID:22573

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HPRT1, hypoxanthine guanine phosphoribosyltransferase

Summary

There are several Molecular Function annotations to propagate, mostly to phosphoglucomutase activity (GO:0004614). This family also enables propagation of a NOT annotation (see 4) below). I was unable to generate a GAF file because in one case, I need to propagate with a parent term (see 2) below), which cannot be done via the current version of PAINT. I would have liked to be able to propagate to all children of the AN2 node, except for the NOT clade (AN9 node), but I do not think I am able to do that with PAINT right now.


Cross references: See SourceForge items 1893061 and 1893082.


Molecular Function: DONE

Propagation:

1) phosphoglucomutase activity (GO:0004614)

Up-propagation to common ancestor node AN100, down-propagation to the following Ref Genome proteins:

HUMAN_ENSG00000165434 MOUSE_MGI:1918224 RAT_1584532 RAT_1583226 CHICK_419052 DANRE_ZDB-GENE-041008-205 HUMAN_ENSG00000169299

because of positive experimental annotations to MOUSE_MGI:97564, YEAST_S0000004891 (PGM3), ECOLI_PHOSPHGLUCMU-MONOMER

2) intramolecular transferase activity, phsophotransferases (GO:0016868)

Note that this is a parent term to the child terms used in the direct, experimental annotations.

Up-propagation to common ancestor node AN318, down-propagation to the following Ref Genome proteins:

RAT_1305221 CHICK_421841 DANRE_ZDB-GENE-041024-13 CAEEL_WBGene00009006 DROME_FBgn0036298 SCHPO_SPAC13C5.05C SCHPO_SPAC1296.01C ARATH_LOCUS NP_568359 DICDI_DDB_G0281789 ARATH_LOCUS NP_177239 ARATH_LOCUS NP_568350 ECOLI_PHOSMANMUT-MONOMER P24175

because of positive experimental annotations to HUMAN_ENSG00000013375, MOUSE_MGI:97566, YEAST_S000000784 (PCM1), ECOLI_PHOSGLUCOSAMINEMUT-MONOMER P31120

3) hypoxanthine phosphoribosyltransferase activity (GO:0004422)

Up-propagation to AN204, down-propagation to the following Ref Genome proteins:

because of positive experimental annotations to HUMAN_ENSG00000165704, MOUSE_MGI:96217, RAT_2826, ECOLI_HYPOXANPRIBOSYLTRAN-MONOMER P0A9M2.


4) NOT phosphoglucomutase activity (GO:0004614) to the PGM5 clade.

Up-propagation to AN9, down-propagation to the following Ref Genome proteins:

HUMAN_ENSG000000204794 RAT_1584213 RAT_1307969 CHICK_427215 DANRE_ENSDARG000000060745

Notes: mouse and human had experimental (IDA) annotations to NOT, but based on protein alignments, can extend the propagation because the active sites have been well characterized. Thus, the alignment of active sites makes the NOT vs. the positive annotations clear. See File:GO EUGENE 2009 pipeline1.ppt for details. Just a note about one protein that has a wildtype site for one active site but mutated for the others: XENTR_ENSXETG000000001670 is in this clade but has the "active" active site at about residue 675 TASHN (where the NOTs have TASHC or TASHS). But, this protein has the other mutated active sites (~1100 FDAD, while active sites have FDGD, and ~1325 has FDY while active sites have YDY).



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