Panther ID:22573
HPRT1, hypoxanthine guanine phosphoribosyltransferase
Summary
There are several Molecular Function annotations to propagate, mostly to phosphoglucomutase activity (GO:0004614). This family also enables propagation of a NOT annotation (see 4) below). I was unable to generate a GAF file because in one case, I need to propagate with a parent term (see 2) below), which cannot be done via the current version of PAINT.
Cross references: See SourceForge items 1893061 and 1893082.
Molecular Function: DONE
Propagation:
1) phosphoglucomutase activity (GO:0004614)
Up-propagation to common ancestor node AN100, down-propagation to the following Ref Genome proteins:
HUMAN_ENSG00000165434
MOUSE_MGI:1918224
RAT_1584532
RAT_1583226
CHICK_419052
DANRE_ZDB-GENE-041008-205
HUMAN_ENSG00000169299
because of positive experimental annotations to MOUSE_MGI:97564, YEAST_S0000004891 (PGM3), ECOLI_PHOSPHGLUCMU-MONOMER
2) phosphoglucomutase activity (GO:0004614)
Propagate to all children of the AN2 node, except for the NOT clade (AN9 node).
RAT_3316
CHICK_424691
DANRE_ZDB-GENE-040426-1245
CAEEL_WBGene00019890
DICDI_DDB_G0288483
SCHPO_SPBC32F12.10
ARATH_LOCUS NP_173732
ARATH_LOCUS NP_177230
based on
HUMAN_ENSG00000079739
MOUSE_MGI:97565
DROME_FBgn0003076
YEAST_S000004711
YEAST_S000001610
ARATH_LOCUS NP_199995
3) intramolecular transferase activity, phsophotransferases (GO:0016868)
Note that this is a parent term to the child terms used in the direct, experimental annotations.
Up-propagation to common ancestor node AN318, down-propagation to the following Ref Genome proteins:
RAT_1305221
CHICK_421841
DANRE_ZDB-GENE-041024-13
CAEEL_WBGene00009006
DROME_FBgn0036298
SCHPO_SPAC13C5.05C
SCHPO_SPAC1296.01C
ARATH_LOCUS NP_568359
DICDI_DDB_G0281789
ARATH_LOCUS NP_177239
ARATH_LOCUS NP_568350
ECOLI_PHOSMANMUT-MONOMER P24175
4) hypoxanthine phosphoribosyltransferase activity (GO:0004422)
Up-propagation to AN204, down-propagation to the following Ref Genome proteins:
because of positive experimental annotations to HUMAN_ENSG00000165704, MOUSE_MGI:96217, RAT_2826, ECOLI_HYPOXANPRIBOSYLTRAN-MONOMER P0A9M2.
5) NOT phosphoglucomutase activity (GO:0004614) to the PGM5 clade.
Up-propagation to AN9, down-propagation to the following Ref Genome proteins:
HUMAN_ENSG000000204794
RAT_1584213
RAT_1307969
CHICK_427215
DANRE_ENSDARG000000060745
Notes: mouse and human had experimental (IDA) annotations to NOT, but based on protein alignments, can extend the propagation because the active sites have been well characterized. Thus, the alignment of active sites makes the NOT vs. the positive annotations clear. See File:GO EUGENE 2009 pipeline1.ppt for details. Just a note about one protein that has a wildtype site for one active site but mutated for the others: XENTR_ENSXETG000000001670 is in this clade but has the "active" active site at about residue 675 TASHN (where the NOTs have TASHC or TASHS). But, this protein has the other mutated active sites (~1100 FDAD, while active sites have FDGD, and ~1325 has FDY while active sites have YDY).
Biological Process: No propagation
Cellular Component: No propagation
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