Phylogenetic Annotation Project
Goal of the Reference Genome Annotation Project
The GO consortium has established the complete annotation of 12 reference genomes as a priority goal. These reference genomes are:
Arabidopsis thaliana, Caenorhabditis elegans, Danio rerio, Dictyostelium discoideum, Drosophila melanogaster, Escherichia coli, Gallus gallus, Homo sapiens, Mus musculus, Rattus norvegicus, Saccharomyces cerevisiae, Schizosaccharomyces pombe
The Reference Genome GO Annotation Team, with representatives from each genome annotation group, will coordinate annotation, facilitate implementation of GO Consortium annotation priorities, provide metrics to assess progress toward the goal of broad and deep annotation of the reference genomes. This group will be responsible for the coordination of the annotation of the twelve reference genomes. This group represents the annotation expertise within the GO consortium and provides key liaisons to the model organism databases the have primary responsibilities for the annotation of the reference genomes.
Reference Genome Annotation Project Summary
Reference Genome Web Page Draft
Progress Reports
- 2008-08-13 RefGen RefGenProgress_2008-09-10
- 2008-08-13 RefGen RefGenProgress_2008-08-13
- 2008-07-18 RefGen RefGenProgress_2008-07-18
- 2008-06-18 RefGen RefGenProgress_2008-06-18
- 2008-06-04 RefGen RefGenProgress_2008-06-04
- 2008-04-20 Second Reference Genome Annotation Meeting, Salt Lake City, UT [Minutes]
- 2007-09-27: First Reference Genome Annotation Meeting, Princeton, NJ [Minutes]
Communication
Reference Genome Mailing list
Conference Calls
Meetings
Electronic jamborees
Annotation Targets
From May 2008
Target Gene List (May 2008-)
Target Gene List August 2006-April 2008
- Access requires your email to be added to the system. Email Pascale if you would like to be added.
- This spreadsheet contains links to separate spreadsheets maintained by each of the reference genome groups.
Procedure for selection of target genes
Procedure for filling Genome-Specific spreadsheets
Gene Annotation
Annotation_pipeline
By Judy, Suzi, Michael
Annotation Quality control
- Annotation QC
- Annotation completion Source Forge tracker [http://sourceforge.net/tracker/?group_id=36855&atid=1040173]
- Reference_Genome_Database_Reports. Those reports are generated with the GOOSE SQL interface and provide lists of potentially mis-annotated genes.
Annotation Consistency Issues
- Annotation consistency: IEA, ISS, IC Usage Discussion: Tanya Berardini, Emily Dimmer, Pascale Gaudet, David Hill, Chris Mungall, Kimberly VanAuken
- Annotation consistency: IDA or IC for processes: Tanya Berardini, Emily Dimmer, Pascale Gaudet, David Hill, Chris Mungall, Kimberly VanAuken, Ruth Lovering
- Annotation consistency: HTP Annotation of high throughput experiments, including microarray data SGD GO HTP guidelines : Stacia Engel, Emily Dimmer, Val Wood, Ruth Lovering
- Annotation consistency: Using IEP, including microarray data, and heat shock protein example: Emily Dimmer, Stacia Engel, Pascale Gaudet, Ruth Lovering, Varsha Khodiyar, Val Wood
- Annotation consistency: x protein binding and with : Becky Foulger, David Hill
- Annotation consistency: xx binding in the context of gene product: example is 'co-enzyme binding' from electronic jamboree : Pascale Gaudet, David Hill, Victoria Petri,
- Annotation consistency: 'Response to' terms: Check that all databases use evidence codes correctly for those terms. Tanya Berardini, Emily Dimmer, Pascale Gaudet, Ruth Lovering
Improving GO terms and definitions
- Annotation consistency: Clarification of oligomerization, dimerization, protein complex assembly: Debby Siegele
- Annotation consistency: chaperone activity definition: clarify the definitions of unfolded/misfolded protein binding and add chaperone activity as a synonym to both of the terms. Also add ‘de novo’ synonym to ‘unfolded protein binding’. Victoria Petri
Misused terms
This page provides a list of often misused terms and (hopefully) an explanation as to how to use the term properly. This information should also be included in the 'comments' of the OBO file.
Variant_annotation
- This page describes how each database handles suration of multiple forms of the same gene
Providing annotations to GOA in taxa other than you MOD's
- Please follow these instructions if you encounter a gene not from your database that you need to annotate.
Gene Annotation wiki pages
- The purpose of these pages are to allow discussions of annotation and orthology issues related to particular genes. The individual gene pages are to be created as needed.
Annotation Progress
Graphical views of the annotations:
Selected refG target sets
- PPOD clusters selected since April 2008
- Manually curated target sets selected before April 2008
All PPOD clusters with at least one object from each of the twelve refG organisms
Reference Genomes Metrics | Metrics: Discussion on annotation progress measurements
Orthology determination
Running P-POD orthology tool on the reference genomes gene set
by Kara Dolinski at Princeton - Nov2007
- This page contains a description of the project and the requirements for providing files
List of potentially problematic families for all vs. all BLAST methods of orthology determination
SOP for determining ortholog (by database)
The purpose of this page is to discuss general principles and problems with establishing orthology between reference genome genes and human disease genes.
Tools for orthology determination
A summary of tools available to identify orthologs.
GFF3 sequence files for reference genome MODs
Reference_Genome_sequence_annotation
Software/database development
The purpose of this page is to discuss features and requirements that would be desirable in a database used to replace the existing Google Spreadsheet system for managing target genes, their annotations and metrics.