Reference Genome Meeting Minutes April 2008

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April 20, 2008

Annotation Progress (Mike Cherry)

  • Number of annotated genes per organism by evidence type (overall)
    • Compare graphs for Sept 2007 and Apr 2008 - overall size and size the same, but IEA decreasing

Discussion: What is effort/person? X-axis is absolute number of genes, which doesn't reflect differences in genome size.

  • Number of annotated genes per organism by evidence code for Reference Genome project
    • majority of genes have experimental evidence codes
  • Discussion:
    • Graph needs outline that indicates "no ortholog". This allows a comparison of the genes present or absent in the reference genome genomes. It will also show which organisms are lagging behind.
    • Number of annotations as a metric?

Annotation Progress (Chris Mungall)

Review Annotation Pipeline proposal (Suzi Lewis)

Step 1: Generation of protein sets (excluding functional RNAs)

    • How to define a coherent set
      • For experimental annotations, want to annotate to isoforms. But for tree building want longest protein produced from a gene. So for ortho sets want a unique protein/gene ID for the "canonical" gene/protein.
      • Heterogeneity in column 2 (gene association file). One suggestion is to add another column. Multiple isoform IDS on one line.

      • Gene Association files (see Annotation of alternate spliceforms) What is current practice?
        • How does UniProt deal with alternate splice forms? Most of the time, there is a 1:1 correspondence between the canonical protein ID and the gene. Uniprot uses canonical identifier followed by -1, -2, etc to indicate isoforms. But sometimes isoforms are so different that they are given separate accessions. In that case, what connects them? have to link out to genomic database.
        • WormBase uses a mixture of gene and protein IDs. (Which is used depends upon how the experiments were done.)
        • MGI

Step 2: Experimental Annotation

Step 3: Inferential Annotation

Step 4: Quality Checks