HPRT1, hypoxanthine guanine phosphoribosyltransferase
There are several Molecular Function annotations to propagate, mostly to phosphoglucomutase activity (GO:0004614). This family also enables propagation of a NOT annotation (see 4) below). I was unable to generate a GAF file because in one case, I need to propagate with a parent term (see 2) below), which cannot be done via the current version of PAINT.
Cross references: See SourceForge items 1893061 and 1893082.
Molecular Function: DONE
1) phosphoglucomutase activity (GO:0004614)
Up-propagation to common ancestor node AN100, down-propagation to the following Ref Genome proteins:
because of positive experimental annotations to MOUSE_MGI:97564, YEAST_S0000004891 (PGM3), ECOLI_PHOSPHGLUCMU-MONOMER
2) phosphoglucomutase activity (GO:0004614)
Up-propagation to common ancestor node AN2, down-propagation to the following Ref Genome proteins. Note that the AN9 node is the NOT clade, and positive propagation to that node should not be done:
because of positive experimental annotations to:
3) intramolecular transferase activity, phsophotransferases (GO:0016868)
Note that this is a parent term to the child terms used in the direct, experimental annotations.
Up-propagation to common ancestor node AN318, down-propagation to the following Ref Genome proteins:
because of positive annotations to HUMAN_ENSG00000013375, MOUSE_MGI:97566, YEAST_S000000784 PCM1, ECOLI_PHOSGLUCOSAMINEMUT-MONOMER P31120.
4) hypoxanthine phosphoribosyltransferase activity (GO:0004422)
Up-propagation to AN204, down-propagation to the following Ref Genome proteins:
Rat Prtfdc1 (RGDID 1310177)
because of positive experimental annotations to HUMAN_ENSG00000165704, MOUSE_MGI:96217, RAT_2826, ECOLI_HYPOXANPRIBOSYLTRAN-MONOMER P0A9M2.
5) ===== phosphoacetylglucosamine mutase activity (GO:0004610) =====
Up-propagation to AN321, down-propagation to the following Ref Genome proteins:
because of positive experimental annotations to HUMAN_ENSG0000001375, MOUSE_MGI:97566, YEAST_S000000784 PCM1.
6) NOT phosphoglucomutase activity (GO:0004614) to the PGM5 clade.
Up-propagation to AN9, down-propagation to the following Ref Genome proteins:
Notes: mouse and human had experimental (IDA) annotations to NOT, but based on protein alignments, can extend the propagation because the active sites have been well characterized. Thus, the alignment of active sites makes the NOT vs. the positive annotations clear. See File:GO EUGENE 2009 pipeline1.ppt for details. Just a note about one protein that has a wildtype site for one active site but mutated for the others: XENTR_ENSXETG000000001670 is in this clade but has the "active" active site at about residue 675 TASHN (where the NOTs have TASHC or TASHS). But, this protein has the other mutated active sites (~1100 FDAD, while active sites have FDGD, and ~1325 has FDY while active sites have YDY).
Biological Process: No propagation
Cellular Component: No propagation
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