Annotation Conf. Call 2015-10-27: Difference between revisions
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'''Choice of paper-motivation''': discussing the use of IGI as evidence code. | '''Choice of paper-motivation''': discussing the use of IGI as evidence code. | ||
When should we use IGI? | When should we use IGI? | ||
: | :- mutation in gene A is rescued by a mutation in gene B | ||
: | :- mutation in gene A is rescued by over-expression in gene | ||
In this paper, how do we capture | In this paper, how do we capture | ||
: | :- the repression of transcriptional activation activity of tbx5a by kctd10? | ||
: | :- the information about has2 | ||
'''Summary of the paper''' (PMID:24430697) | '''Summary of the paper''' (PMID:24430697) | ||
- mutants in kctd10 show heart defects (heart looping, atrioventricular canal development) | - mutants in kctd10 show heart defects (heart looping, atrioventricular canal development) | ||
Note: phenotypes determined by | Note: phenotypes determined by | ||
: | :- analysis of heart morphology | ||
: | :- gene expression analysis of genes known to be expressed in specific part of the heart). | ||
[Fig 1, 2, 3] | [Fig 1, 2, 3] | ||
Line 24: | Line 24: | ||
- in vitro experiments [Fig5]: | - in vitro experiments [Fig5]: | ||
: | :- luciferase reporter assay | ||
kctd10 inhibits tbx5 transcriptional activity | :kctd10 inhibits tbx5 transcriptional activity | ||
: | :- Co-IP ; Pull down assay: | ||
tbx5a and kctd10 physically interact with each other. | :tbx5a and kctd10 physically interact with each other. | ||
==Minutes== | ==Minutes== | ||
[[Category:Annotation Working Group]] | [[Category:Annotation Working Group]] |
Revision as of 19:16, 26 October 2015
Meeting URL: https://bluejeans.com/993661940
Agenda
Annotation Consistency Exercise
Annotation consistency paper picked by ZFIN- PMID:24430697
Choice of paper-motivation: discussing the use of IGI as evidence code. When should we use IGI?
- - mutation in gene A is rescued by a mutation in gene B
- - mutation in gene A is rescued by over-expression in gene
In this paper, how do we capture
- - the repression of transcriptional activation activity of tbx5a by kctd10?
- - the information about has2
Summary of the paper (PMID:24430697) - mutants in kctd10 show heart defects (heart looping, atrioventricular canal development) Note: phenotypes determined by
- - analysis of heart morphology
- - gene expression analysis of genes known to be expressed in specific part of the heart).
[Fig 1, 2, 3]
- downregulation of tbx5a or has 2 (using MO) rescue kctd10 mutant heart phenotypes. [Fig 4]
- in vitro experiments [Fig5]:
- - luciferase reporter assay
- kctd10 inhibits tbx5 transcriptional activity
- - Co-IP ; Pull down assay:
- tbx5a and kctd10 physically interact with each other.