Annotation Conf. Call 2015-10-27

Meeting URL: https://bluejeans.com/993661940

Agenda

Annotation Consistency Exercise

Annotation consistency paper picked by ZFIN- PMID:24430697

Choice of paper-motivation: discussing the use of IGI as evidence code. When should we use IGI?

Indented line - mutation in gene A is rescued by a mutation in gene B

Indented line - mutation in gene A is rescued by over-expression in gene

In this paper, how do we capture

Indented line - the repression of transcriptional activation activity of tbx5a by kctd10?

Indented line - the information about HAS2

Summary of the paper (PMID:24430697) - mutants in kctd10 show heart defects (heart looping, atrioventricular canal development) Note: phenotypes determined by

Indented line - analysis of heart morphology

Indented line - gene expression analysis of genes known to be expressed in specific part of the heart).

[Fig 1, 2, 3]

- downregulation of tbx5a or has 2 (using MO) rescue kctd10 mutant heart phenotypes. [Fig 4]

- in vitro experiments [Fig5]:

Indented line - luciferase reporter assay

kctd10 inhibits tbx5 transcriptional activity

Indented line - Co-IP ; Pull down assay:

tbx5a and kctd10 physically interact with each other.