Annotation Conf. Call 2022-11-01

Agenda and Minutes

Meetings and Announcements

Noctua

• Next regularly scheduled Noctua outage:
  • Thursday, November 10th
  • Outage tasks

Fall GOC Meeting Follow-Up

• Survey - has everyone replied?

GO Release Pipeline

• November release will start this week
  • Encourage people to check #release-pipeline channel on Slack for up-to-date info

Using PRO in GO Annotation

• Presentation from Darren Natale on using PRO to create modified forms of proteins for GO annotation

Ontology

• Term obsoletions vs term merges
  • Chris prefers we obsolete terms and use REPLACED BY rather than merging terms, which seems to confuse users.
  • Are there objections in the group?
  • Does this work with groups' current pipelines and tools?

Annotation

Annotation Questions on Slack Channel

Annotating to transcription coactivator term (Antonia)

• ZMYND8 directly binds the P-TEFb complex and stimulates phosphorylation of RNA pol II CTD to stimulate transcription. The authors call it a coactivator - can I annotate to this term or is P-TEFb function downstream?

Annotation of cortical proteins attached to the cell membrane (Val)

• How would you curate the location of a proteins that is described as "cortical", acts as a "membrane adaptor" and attaches to the "plasma membrane" by a prenylation signal?
• "Here we describe a cortical protein, mod5p, that regulates the dynamic behaviour of tea1p. In mod5Delta cells, tea1p is efficiently transported on microtubules to cell tips but fails to anchor properly at the cortex and thus fails to accumulate to normal levels. mod5p contains a signal for carboxy-terminal prenylation and in wild-type cells is associated with the plasma membrane at cell tips."
• I think in the discussion we said the "plasma membrane". and "cell cortex" were mutually exclusive, but researchers do not seem to make the same distinction?

Annotation error reports and annotation review

• Please remember to keep checking the reports generated by the pipeline and address any errors
• Also check annotation review tickets assigned to you/your group in go-annotation repo on github
• Questions about either - please bring to the meeting

Attendees

• On call: Antonia, Colin, Darren, Deborah, Dmitry, Dustin, Edith, Giulia, Karen, Kimberly, Leonore, Li, Malcolm, Pascale, Patrick, Peter, Petra, Raymond, Rob, Seth, Sierra, Sridhar, Suzi, Val