Annotation Conf. Call 2022-11-15

Agenda and Minutes

Meetings and Announcements

Noctua

• Next regularly scheduled Noctua outage:
  • Thursday, December 1st and December 15th
  • Outage tasks

Spring GOC Meeting Plans

• Survey - please reply
  • Padua, Italy - late April, before the ISB meeting
    • Another Italy option - the two days after the ISB (this was not on the original survey, but is now also an option) - email Pascale if you have strong feelings one way or the other wrt before/after the ISB meeting
  • Baltimore, MD - late May/early June (two options)

GO Release Pipeline

• November release out on November 3rd
  • Encourage people to check #release-pipeline channel on Slack for up-to-date info
• If your group changes any practice that would alter significantly the number of annotations, please let Pascale know

Using PRO in GO Annotation

• Presentation from Darren Natale on using PRO to create modified forms of proteins for GO annotation

Annotation

Annotation Reviews

• Histone modifications
• Extrinsic component of membrane

Annotation Questions on Slack Channel

Annotating to transcription coactivator term (Antonia)

• ZMYND8 directly binds the P-TEFb complex and stimulates phosphorylation of RNA pol II CTD to stimulate transcription. The authors call it a coactivator - can I annotate to this term or is P-TEFb function downstream?

Annotation of cortical proteins attached to the cell membrane (Val)

• How would you curate the location of a proteins that is described as "cortical", acts as a "membrane adaptor" and attaches to the "plasma membrane" by a prenylation signal?
• "Here we describe a cortical protein, mod5p, that regulates the dynamic behaviour of tea1p. In mod5Delta cells, tea1p is efficiently transported on microtubules to cell tips but fails to anchor properly at the cortex and thus fails to accumulate to normal levels. mod5p contains a signal for carboxy-terminal prenylation and in wild-type cells is associated with the plasma membrane at cell tips."
• I think in the discussion we said the "plasma membrane". and "cell cortex" were mutually exclusive, but researchers do not seem to make the same distinction?

Annotation error reports and annotation review

• Please remember to keep checking the reports generated by the pipeline and address any errors
• Also check annotation review tickets assigned to you/your group in go-annotation repo on github
• Questions about either - please bring to the meeting

Attendees

• On call: David, Deborah, Dmitry, Dustin, Edith, Giulia, Harold, Karen, Kimberly, Li, Pascale, Patrick, Raymond, Rob, Seth, Suzi