Annotation Conf. Call December 10, 2013
Agenda
ubiquitin hydrolase
Pascale
- Confusion between use of:
GO:0004221 ubiquitin thiolesterase activity
and
GO:0004843 ubiquitin-specific protease activity
1) ubiquitin thiolesterase activity: Catalysis of the reaction: ubiquitin C-terminal thioester + H2O = ubiquitin + a thiol (mapped to EC:3.1.2.15)
2) ubiquitin-specific protease activity: Catalysis of the hydrolysis of various forms of polymeric ubiquitin sequences (not mapped to any EC term).
However my understanding is that, although the reaction is sulfur-dependent, it's still a protease, and NOT a thiolesterase. Is this right ?
Those annotations are also in the following InterPro families:
IPR001394 IPR001578 IPR006615 IPR018200
I have the feeling the ubiquitin thiolesterase activity is not a real reaction. See http://sourceforge.net/p/geneontology/ontology-requests/10519/ for request to obsolete. I am waiting for the Swiss-Prot enzyme expert for input (Kristian Axelsen)