PTHR11289

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Revision as of 15:53, 21 March 2011 by Livstone (talk | contribs) (Removed passage that had been accidentally duplicated)
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This is a list of questions that arose while PAINTing PTHR11289.

Questions for MOD curators

Molecular Function

Human (P51587)

  • Question: Can it be confirmed that PMID: 9619837 shows that BRCA2 has transcription factor activity? I cannot see that. (emailed Rachael Jan 17, 2011 -PG)
    • Curator answer: Yes, there doesn't appear to be experimental evidence for the transcription activator activity, it's more of a TAS. However, in PMID:9126734 there is evidence for this term, so I have updated the annotation to this paper. I have also added annotations to BRCA2 for histone acetyltransferase activity from PMID: 9619837, as these were missing. - Rachael (GOA) Jan 18, 2011
  • Question: Centrosome duplication annotation IDA from PMID: 17286961: Should this not be an IMP? (" GFP–CLS prevents endogenous BRCA2 from localizing to the centrosome (middle panel).") Would you be comfortable propagating this information?
    • Curator answer: So the CLS (centrosome localisation signal) of BRCA2 is preventing endogenous BRCA2 from localising to the centrosome thus causing abnormal centrosome duplication, so in this case the GFP-CLS is acting as an inhibitor of BRCA2, so I have updated the annotation to IMP.
    • Curator answer: They do a co-IP on Page 37, it says: "We thus performed a co-precipitation analysis that showed that BRCA2 associates with gamma-tubulin. BRCA2 was immunoprecipitated from HeLa cells, and analysis of anti-BRCA2 immunoprecipitates with anti-gamma-tubulin demonstrated the presence of gamma-tubulin in the immunoprecipitated complexes (Fig. 2C, left panel). In reciprocal experiments, a similar immunoblot analysis of anti-gamma-tubulin immunoprecipitates for the presence of BRCA2 confirmed the interaction of the two proteins...These results indicate that there is a specific direct or indirect interaction between gamma-tubulin and BRCA2."

Rat (O35923)

  • Question: Response to estrogen stimulus: Can this be confirmed? (PMID: 14583453). It seems like the gene is a target of the estrogen response, not a mediator.
    • Curator answer:The definitions of the 'response to' terms are far broader in scope than a role of mediation. The annotation will remain as is.
  • Question: Idem for Response to nutrients.
    • Curator answer:The annotation will remain as is.

Questions for ontology developers

  • Question: BRCA2-MAGE-D1 complex ; GO:0033593: Is this a real complex? If so, why is MAGE-D2 protein not annotated? (SF# 3220799)
    • Curator answer: