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PTHR12027 tree annotation discussion: wnt

This is a list of questions that arose while PAINTing the Wnt family. Please comment liberally, and please sign your comments. -Mike

Questions for MOD curators

Molecular Function


GO:0004871 "signal transducer activity" has the comment "Ligands do NOT have the molecular function 'signal transducer activity'." Can you please clarify the 4871 IGI on wnt-2?

I removed the manual IGI annotation to signal transducer activity, but also noticed that we have the same annotation in WB as an IEA via the InterPro2GO mapping. This annotation also gets applied to the other C. elegans Wnts via the same mapping.

InterPro:IPR005816 Secreted growth factor Wnt protein > GO:signal transducer activity ; GO:0004871

There are some others in the InterPro2GO mappings file that should also be checked:

InterPro:IPR005817 Wnt superfamily > GO:signal transducer activity ; GO:0004871 InterPro:IPR009139 Wnt-1 protein > GO:signal transducer activity ; GO:0004871 (and many other Wnt-number proteins share this mapping)

I'll submit this as a SourceForge item.



Please verify the Wnt9a GO:0043028 "caspase regulator activity" annotation. Is this an actual molecular function of Wnt9a or just a downstream effect?

Cellular Component

  • All: There are multiple annotations to "plasma membrane." Do you agree that this is valid for a ligand?
  • All: There is 1 annotation to GO:0045121 "membrane raft" (Drosophila wingless, PMID 15166250). I've propagated it to the entire Wnt1/Wnt3 clade, but haven't found any other literature to support such an annotation for any Wnt. Comments would be appreciated.

My understanding is that membrane rafts are quite specialised structures, so I would not annotate to the whole clade if there is no literature to support this in other species -- Varsha

Biological process

  • Danio: Should either of the GO:0030178 "negative regulation of Wnt receptor signaling pathway" IGI's be to the child term GO:0090090 "negative regulation of canonical Wnt receptor signaling pathway"?
  • All: Even though there is a major reconfiguration of the transcription part of the ontology underway, please consider using more specific terms whenever possible. Examples:
	GO:0010628 "positive regulation of gene expression"	OR	GO:0045449 "regulation of transcription"
		GO:0043193 "positive regulation of gene-specific transcription"
			GO:0010552 "positive regulation of gene-specific transcription from RNA polymerase II promoter"

or the corresponding negative regulation terms if appropriate.

  • Mouse: Do you recommend propagating the Wnt10a GO:0014033 "neural crest cell differentiation" IDA from PMID 17286598 (MGI 3712614) to the rest of the Wnt10a's? I can't access the fulltext paper.
  • Mouse: Should the Wnt7a GO:0016055 "Wnt receptor signaling pathway" IDA from PMID 19497282, "Wnt7a activates the planar cell polarity pathway to drive the symmetric expansion of satellite stem cells," actually be to GO:0060071 "Wnt receptor signaling pathway, planar cell polarity pathway"?
  • Rat and Mouse: Mouse Wnt7a has an annotation to GO:0050770 "regulation of axonogenesis." Rat Wnt7b has an annotation to GO:0032536 "regulation of cell projection size" from PMID 18177422; the abstract of this paper states, "Ectopic wnt7b overexpression was sufficient to rescue neurite outgrowth in NGF-treated p53-silenced PC12 cells." Is this sufficient to generalize "regulation of axonogenesis" to both Wnt 7a and -7b? Put another way, are PC12 "neurite outgrowths" sufficiently axon-like that this is probably the same process?
  • Zebrafish: wnt8a and -8b have annotations to GO:0001654 "eye development." Please specify that this is "camera-type" (GO:0043010) and consider more specific child terms such as "embryonic" (GO:0031076), "morphogenesis" (GO:0048593), or both (GO:0048596).
  • Mouse: For Wnt10b GO:0043586 "tongue development," would you prefer the child term GO:0061196 "fungiform papilla development"? The reference (PMID 17284610) states "Shh expression in fungiform papillae and formation of normal mature fungiform papillae depend on signaling through Wnt and beta-catenin."
  • Mouse: For Wnt6, instead of GO:0048754 "branching morphogenesis of a tube" from PMID 11948913, would you prefer GO:0001658 "branching involved in ureteric bud morphogenesis"? Instead of GO:0035148 "tube formation," would you prefer GO:0072079 "nephron tubule formation"?
  • Mouse: Please verify the Wnt1 GO:0001658 "branching involved in ureteric bud morphogenesis" IGI from PMID 16054034. The paper says that Wnt1 can substitute fror Wnt9b in urogenital development, but does that mean that that is the natural role of Wnt1?
  • Mouse: Wnt7b has an IMP to GO:0032364 "oxygen homeostasis" from PMID 18367557. Is this due to its lung development phenotype? Is Wnt7b directly involved in oxygen homeostasis, or is this a downstream effect?
  • Mouse Wnt7b: Instead of GO:0042476 "odontogenesis," would you prefer GO:0042475 "odontogenesis of dentine-containing tooth"?
  • Danio: Does PMID 18798004, "A role for planar cell polarity signaling in angiogenesis," justify an wnt5 annotation to GO:0060071 "Wnt receptor signaling pathway, planar cell polarity pathway"?
  • Danio: Instead of the wnt5b GO:0010172 "embryonic body morphogenesis" annotation from PMID 9598355, would you prefer GO:0035121 "tail morphogenesis," or were you trying to stress the "embryonic" aspect of the annotation? Does 35121 imply that the process is embryonic? Similarly, can you specify an organ/tissue for the wnt8b GO:0009880 "embryonic pattern specification" annotation from PMID 15366005?
  • Worm: Is signaling through Ror a new pathway? See PMID 19855022.
  • Mouse: Wnt9b has IMP's to GO:0048701 "embryonic cranial skeleton morphogenesis" and GO:0060021 "palate development," both from PMID 16054034. Do you want to request some sort of cross-product, like "embryonic palate morphogenesis"?
  • Mouse: Would you like to make an annotation to GO:0035567 "non-canonical Wnt receptor signaling pathway" for Wnt11 based on PMID 17767158, "Modulation of morphogenesis by noncanonical Wnt signaling requires ATF/CREB family-mediated transcriptional activation of TGFbeta2"?
  • Mouse: Please double-check the Wnt4 GO:0072034 "positive regulation of renal vesicle formation" annotation from PMID 11507767. The paper seems to be about Wnt2.

Questions for ontology curators

  • Is there a plan to make GO:0005110 "frizzled-2 binding" a child of GO:0005109 "frizzled binding" as described in SF # 3025937?
  • Does GO:0060070 "canonical Wnt receptor signaling pathway" has_part "regulation of gene expression" or something like it?
  • Should GO:0060071 "Wnt receptor signaling pathway, planar cell polarity pathway," defined as "The series of molecular signals initiated by binding of a Wnt protein to a receptor on the surface of the target cell where activated receptors signal via downstream effectors including C-Jun N-terminal kinase (JNK) to modulate cytoskeletal elements and control cell polarity," has_part GO:0007254 "JNK cascade" or something like it?
  • Should there be a more direct relationship between GO:0046330 "positive regulation of JNK cascade" and GO:0043507 "positive regulation of JUN kinase activity"? They are both children of GO:0046328 "regulation of JNK cascade."
  • There are some "development" terms that have "embryonic" children, and some that don't. For those that don't, is it assumed/understood that the processes occur in the embryo? Would these be the sort of terms that it should be possible to auto-request?
    • The lack of embryonic children under development terms simply means that we have not added them. It does not assume that the process always or completely occurs in the embryo. -dph

Obsolete information that was on this page:

PTHR12027 (subfamily): Jan 2010 targets for lung branching morphogenesis project

This is for the lung branching morphogenesis annotation project

  • CAEEL|WB=WBGene00000858|UniProtKB=P34889
  • CHICK|ENTREZ=374243|UniProtKB=Q98SN7
  • CHICK|ENTREZ=395703|UniProtKB=Q9YGX6
  • CHICK|ENTREZ=417769|UniProtKB=A0M8U2
  • CHICK|ENTREZ=418154|UniProtKB=Q3L255
  • DANRE|ZFIN=ZDB-GENE-030717-2|UniProtKB=Q8AY89
  • DANRE|ZFIN=ZDB-GENE-060328-3|UniProtKB=A7E271
  • DANRE|ZFIN=ZDB-GENE-060824-6|UniProtKB=Q1L130
  • DANRE|ZFIN=ZDB-GENE-980526-416|UniProtKB=Q92048
  • DANRE|ZFIN=ZDB-GENE-980526-87|UniProtKB=Q92050
  • DROME|FB=FBgn0010194|UniProtKB=P28466
  • HUMAN|ENSEMBL=ENSG00000105989|UniProtKB=P09544
  • HUMAN|ENSEMBL=ENSG00000111186|UniProtKB=Q9H1J7
  • HUMAN|ENSEMBL=ENSG00000114251|UniProtKB=P41221
  • HUMAN|ENSEMBL=ENSG00000134245|UniProtKB=Q93097
  • MOUSE|MGI=MGI=1261834|UniProtKB=O70283
  • MOUSE|MGI=MGI=98954|UniProtKB=P21552
  • MOUSE|MGI=MGI=98958|UniProtKB=P22725
  • MOUSE|MGI=MGI=98959|UniProtKB=P22726
  • RAT|RGD=621346|UniProtKB=Q2IBD1
  • RAT|RGD=628850|UniProtKB=Q8CFB3
  • RAT|RGD=69250|UniProtKB=Q9QXQ7
  • RAT|RGD=69346|UniProtKB=Q9QXK5